VMAT2 Polyclonal antibody

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• KD/KO Validated

VMAT2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 20873-1-AP

Synonyms

SLC18A2

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Validation Data Gallery

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  WB analysis of Jurkat using 20873-1-AP

  WB result of VMAT2 antibody (20873-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VMAT2 transfected Jurkat cells.

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  WB analysis of SGC-7901 using 20873-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 20873-1-AP (VMAT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20873-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20873-1-AP (VMAT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 20873-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20873-1-AP (VMAT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 20873-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using VMAT2 antibody (20873-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SGC-7901 cells, Jurkat cells
• Positive IHC detected in: mouse brain tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 5 publications below

Product Information

20873-1-AP targets VMAT2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VMAT2 fusion protein Ag14971
• Full Name: solute carrier family 18 (vesicular monoamine), member 2
• Calculated Molecular Weight: 514 aa, 56 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: BC108928
• Gene Symbol: VMAT2
• Gene ID (NCBI): 6571
• RRID: AB_10858619
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VMAT2 is a vesicular monoamine transporter that is responsible for the uptake and storage of monoamines (dopaine, norepinephrine, epinephrine, histamine and serotonin) in neurons, endocrine cells, and tumors deriving from these cells. Histamine-producing endocrine tumors (gastric carcinoids) expressed VMAT2 almost exclusively, thus VMAT2 is useful in classification of neuroendocrine tumors. Reduced expression of VMAT2 has often been observed in Parkinson's diseased (PD) brain, and detection of VMAT2 can be used to reflect the 4-(2-aminoethyl)benzene-1,2-diol neuron loss and predict the disease process. Several forms of VMAT2 can be observed upon the glycosylation: 45 kDa band corresponds to the native (deglycosylated) form, and the 55 and 75 kDa bands to glycosylated forms (PMID: 17582657).

Publications

Species	Application	Title
mouse	WB,IF	Nature; Reversing a model of Parkinson's disease with in situ converted nigral neurons.; Authors - Hao Qian
human	IF	Nat Genet; Identification of TMEM230 mutations in familial Parkinson's disease.; Authors - Han-Xiang Deng
human	WB	Dis Model Mech; A Matrigel-based 3D construct of SH-SY5Y cells models the α-synuclein pathologies of Parkinson's disease.; Authors - Zhao-Feng Li
human	IF	Front Neurosci; Deregulation of mTORC1-TFEB axis in human iPSC model of GBA1-associated Parkinson's disease; Authors - Fahad Mubariz
rat	WB	Biol Res; Effects of latroeggtoxin-VI on dopamine and α-synuclein in PC12 cells and the implications for Parkinson's disease; Authors - Dianmei Yu
mouse	IF	Mol Neurodegener; Pathological characteristics of axons and alterations of proteomic and lipidomic profiles in midbrain dopaminergic neurodegeneration induced by WDR45-deficiency; Authors - Panpan Wang