VLDLR Polyclonal antibody

• Featured Product
• KD/KO Validated

VLDLR Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 19493-1-AP

Synonyms

VLDL-R, VLDL receptor, Very low-density lipoprotein receptor

---

Validation Data Gallery

• 

  WB analysis using 19493-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 19493-1-AP (VLDLR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 19493-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19493-1-AP (VLDLR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 19493-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VLDLR antibody (19493-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Tested Applications

• Positive WB detected in: MCF-7 cells, mouse ovary tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 1 publications below

Product Information

19493-1-AP targets VLDLR in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, chicken, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: very low density lipoprotein receptor
• Calculated Molecular Weight: 96 kDa
• Observed Molecular Weight: 96 kDa, 160 kDa
• GenBank Accession Number: NM_003383
• Gene Symbol: VLDLR
• Gene ID (NCBI): 7436
• RRID: AB_10644342
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VLDLR binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. VLDLR binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation (cytoplasmic domain of low-density lipoprotein receptor). For glycosylated, the MW of VLDLR migrates to ~160kd.

Publications

Species	Application	Title
zebrafish	WB	Neuron; Molecular microcircuitry underlies functional specification in a basal ganglia circuit dedicated to vocal learning.; Authors - Hilliard Austin T AT
mouse	WB	Food Funct; Monounsaturated fatty acid-enriched olive oil exacerbates chronic alcohol-induced hepatic steatosis and liver injury in C57BL/6J mice; Authors - Rui Guo
rat	WB	Front Pharmacol; Protective Effect of Patchouli Alcohol Against High-Fat Diet Induced Hepatic Steatosis by Alleviating Endoplasmic Reticulum Stress and Regulating VLDL Metabolism in Rats.; Authors - Xue Wu
mouse	WB	PLoS One; Gut microbiota and butyrate contribute to nonalcoholic fatty liver disease in premenopause due to estrogen deficiency.; Authors - Limin Liu
mouse	WB	Front Nutr; Dietary camellia seed oil attenuates liver injury in mice chronically exposed to alcohol; Authors - Rui Guo
human	WB,IF	Front Oncol; VLDLR disturbs quiescence of breast cancer stem cells in a ligand-independent function; Authors - Mengying Yang ; KD Validated