VGLUT2 Polyclonal antibody

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  WB analysis using 25261-1-AP

  unboiled rat brain tissue were subjected to SDS PAGE followed by western blot with 25261-1-AP (VGLUT2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 25261-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25261-1-AP (VGLUT2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

VGLUT2 Polyclonal Antibody for WB, ELISA

Cat No. 25261-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

SLC17A6, Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, DNPI

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Tested Applications

• Positive WB detected in: unboiled rat brain tissue, fetal human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25261-1-AP targets VGLUT2 in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_020346
• Gene Symbol: VGLUT2
• Gene ID (NCBI): 57084
• RRID: AB_2879992
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P2U8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VGLUT2, also known as SLC17A6, belongs to the major facilitator superfamily. VGLUT2 is a multifunctional transporter that transports phosphate at the plasma membrane and glutamate in synaptic vesicles (PMID:33440152, 11432869). VGLUT2 is involved in neurotransmitter loading into synaptic vesicle (PMID: 11698620). VGLUT2 is predominantly expressed in adult and fetal brain, with highest expression in the medulla, substantia nigra, subthalamic nucleus, and thalamus, and low levels in the cerebellum and hippocampus (PMID:10820226).