Anti-Mouse VEGFR2/CD309 Rabbit Recombinant Antibody, PBS Only

VEGFR2/CD309 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240214F12

Cat no : 98027-1-PBS

Synonyms

Kdr, Krd 1, Flk1, Flk 1, 6130401C07

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Validation Data Gallery

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  FC experiment of bEnd.3 using 98027-1-RR (same clone as 98027-1-PBS)

  1x10^6 bEnd.3 cells were surface stained with 0.25 ug Anti-Mouse VEGFR2/CD309 Rabbit Recombinant Antibody (98027-1-RR, Clone: 240214F12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 98027-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98027-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98027-1-RR against Mouse VEGFR-2/Kdr were performed. The affinity constant is 2.92 nM.

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Product Information

The immunogen of 98027-1-PBS is VEGFR2/CD309 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: kinase insert domain protein receptor
• Calculated Molecular Weight: 150KD
• GenBank Accession Number: NM_010612.2
• Gene Symbol: VEGFR-2
• Gene ID (NCBI): 16542
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

VEGFR2, also named as CD309, KDR and FLK1, is a receptor for VEGF or VEGFC. VEGFR2 which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. VEGFR2 functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. Mutations of this gene are implicated in infantile capillary hemangiomas.