VEGFR-1/FLT-1 Polyclonal antibody, PBS Only

VEGFR-1/FLT-1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13687-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HEK-293 using 13687-1-AP (same clone as 13687-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13687-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 13687-1-AP (same clone as 13687-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13687-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 13687-1-AP (same clone as 13687-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13687-1-PBS in a different storage buffer formulation.

• 

  IP experiment of A549 using 13687-1-AP (same clone as 13687-1-PBS)

  IP result of anti-VEGFR-1/FLT-1 (IP:13687-1-AP, 5ug; Detection:13687-1-AP 1:500) with A549 cells lysate 3300ug. This data was developed using the same antibody clone with 13687-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 13687-1-AP (same clone as 13687-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13687-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 13687-1-AP (same clone as 13687-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13687-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 13687-1-AP (same clone as 13687-1-PBS)

  Immunofluorescent analysis of HeLa cells using 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13687-1-PBS in a different storage buffer formulation.

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Product Information

13687-1-PBS targets VEGFR-1/FLT-1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VEGFR-1/FLT-1 fusion protein Ag4558
• Full Name: fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
• Calculated Molecular Weight: 1338 aa, 151 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC039007
• Gene Symbol: VEGFR-1/FLT-1
• Gene ID (NCBI): 2321
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Vascular endothelial growth factor receptor-1 (VEGFR-1, FLT-1) is a receptor tyrosine kinase belonging to the VEGFR family. VEGF is a key regulator of physiological angiogenesis and has also been implicated in pathological angiogenesis associated with tumors, intraocular neovascular disorders, and other conditions. The biological effects of VEGF are mediated by VEGFR-1 and VEGFR-2. Both the two receptors have seven immunoglobulin-like repeats in the extracellular domain, a single transmembrane region and a tyrosine kinase domain. VEGFR-1 binds VEGFA, PIGF, and VEGFB, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Two isoforms of VEGFR-1 exist, a full-length transmembrane form and a short soluble form (sVEGFR-1) consisting of only the extracellular ligand-binding domain. (PMID: 12778165; 17109193; 8806634)