VEGFA Recombinant antibody
VEGFA Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
7H10
Cat no : 81323-2-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF-7 cells, T-47D cells, A431 cells, MDA-MB-231 cells, HUVEC cells, RAW 264.7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
81323-2-RR targets VEGFA in WB, ELISA applications and shows reactivity with Human, mouse samples.
Tested Reactivity | Human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | VEGFA fusion protein Ag13500 |
Full Name | vascular endothelial growth factor A |
Calculated Molecular Weight | 412 aa, 46 kDa |
Observed Molecular Weight | 40 kDa |
GenBank Accession Number | BC065522 |
Gene Symbol | VEGFA |
Gene ID (NCBI) | 7422 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ).
Protocols
Product Specific Protocols | |
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WB protocol for VEGFA antibody 81323-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |