VE-cadherin/CD144 Monoclonal antibody, PBS Only

VE-cadherin/CD144 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

4F9B10

Cat no : 66804-1-PBS

Synonyms

VE-cadherin, CDH5, CD144, Cadherin 5, 7B4 antigen

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Validation Data Gallery

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  WB analysis of human placenta using 66804-1-Ig (same clone as 66804-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66804-1-Ig (VE-cadherin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66804-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66804-1-Ig (same clone as 66804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66804-1-Ig (VE-cadherin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66804-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66804-1-Ig (same clone as 66804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66804-1-Ig (VE-cadherin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66804-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66804-1-Ig (same clone as 66804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66804-1-Ig (VE-cadherin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66804-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66804-1-Ig (same clone as 66804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66804-1-Ig (VE-cadherin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66804-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66804-1-Ig (same clone as 66804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66804-1-Ig (VE-cadherin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66804-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66804-1-Ig (same clone as 66804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66804-1-Ig (VE-cadherin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66804-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 66804-1-Ig (same clone as 66804-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using VE-cadherin antibody (66804-1-Ig, Clone: 4F9B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66804-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HUVEC using 66804-1-Ig (same clone as 66804-1-PBS)

  Immunofluorescent analysis of un-fixed HUVEC cells using VE-cadherin/CD144 antibody (66804-1-Ig, Clone: 4F9B10 ) at dilution of 1:1500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66804-1-PBS in a different storage buffer formulation.

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Product Information

66804-1-PBS targets VE-cadherin/CD144 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: VE-cadherin/CD144 fusion protein Ag27501
• Full Name: cadherin 5, type 2 (vascular endothelium)
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 125 kDa, 100 kDa
• GenBank Accession Number: NM_001795
• Gene Symbol: VE-cadherin
• Gene ID (NCBI): 1003
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609).