VDAC3 Polyclonal antibody

VDAC3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 14451-1-AP

Synonyms

HD VDAC3, hVDAC3, VDAC 3, VDAC3

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Validation Data Gallery

• 

  WB analysis of mouse heart using 14451-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14451-1-AP (VDAC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14451-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14451-1-AP (VDAC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14451-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14451-1-AP (VDAC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14451-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14451-1-AP (VDAC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 14451-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14451-1-AP (VDAC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14451-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14451-1-AP (VDAC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human osteosarcoma using 14451-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14451-1-AP (VDAC3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human osteosarcoma using 14451-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14451-1-AP (VDAC3 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue, human brain tissue, human heart tissue, mouse brain tissue
• Positive IHC detected in: human liver cancer tissue, human osteosarcoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 2 publications below

Product Information

14451-1-AP targets VDAC3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VDAC3 fusion protein Ag5960
• Full Name: voltage-dependent anion channel 3
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC056870
• Gene Symbol: VDAC3
• Gene ID (NCBI): 7419
• RRID: AB_2212351
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VDAC3 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules.

Publications

Species	Application	Title
human	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
mouse	WB	Mol Ther Nucleic Acids; Salvia miltiorrhiza-Derived Sal-miR-58 Induces Autophagy and Attenuates Inflammation in Vascular Smooth Muscle Cells.; Authors - Yan Qin
human	IHC	Oncotarget; Proteomics-based identification of VDAC1 as a tumor promoter in cervical carcinoma.; Authors - Changlin Zhang
	WB	J Cell Sci; Doublet 7 shortening, doublet 5-preferential poly-Glu reduction, and beating stall of sperm flagella in Ttll9-/- mice.; Authors - Alu Konno
rat	WB	Int J Mol Med; Herceptin induces ferroptosis and mitochondrial dysfunction in H9c2 cells.; Authors - Lei Sun
human	WB	Front Aging Neurosci; The Protective Role of Mitochondrial Ferritin on Erastin-Induced Ferroptosis.; Authors - Yue-Qi Wang

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