VASH2 Monoclonal antibody, PBS Only

VASH2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A11D10

Cat no : 67753-1-PBS

Synonyms

FLJ12505, RP11 275G3.1, VASH2, VASHL, vasohibin 2, Vasohibin like protein

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Validation Data Gallery

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  WB analysis using 67753-1-Ig (same clone as 67753-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67753-1-Ig (VASH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67753-1-Ig (same clone as 67753-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67753-1-Ig (VASH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 67753-1-Ig (same clone as 67753-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 67753-1-Ig (same clone as 67753-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67753-1-Ig (same clone as 67753-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67753-1-Ig (same clone as 67753-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67753-1-Ig (same clone as 67753-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67753-1-Ig (same clone as 67753-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

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Product Information

67753-1-PBS targets VASH2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: VASH2 fusion protein Ag29985
• Full Name: vasohibin 2
• Calculated Molecular Weight: 355 aa, 40 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC028194
• Gene Symbol: VASH2
• Gene ID (NCBI): 79805
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

VASH2, also named as vasohibin 2, is an important pro-angiogenesis factor in solid tumor. It has been reported that VASH2 is expressed in mononuclear cells mobilized from bone marrow to promote angiogenesis. VASH2 also plays a key role in axon formation. VASH2 is localized as a nuclear type(with 311 amino acid residues) and cytoplasmic type (with 355 amino acid residues and low abundance). Cytoplasmic VASH2 is associated with carcinoma angiogenesis, while nuclear VASH2 may be associated with cell proliferation. 67753-1-Ig antibody recognizes both the nuclear and cytoplasmic isoforms. (PMID:23615928; 19204325; 31235911; 26177649)