VASH2 Monoclonal antibody

VASH2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A11D10

Cat no : 67753-1-Ig

Synonyms

FLJ12505, RP11 275G3.1, VASH2, VASHL, vasohibin 2, Vasohibin like protein

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Validation Data Gallery

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  WB analysis using 67753-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67753-1-Ig (VASH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis using 67753-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67753-1-Ig (VASH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 67753-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 67753-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67753-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67753-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67753-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67753-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SKOV-3 cells, BGC-823 cells, L02 cells, HepG2 cells, HUVEC cells, HSC-T6 cells, NIH/3T3 cells, SGC-7901 cells, MKN-45 cells
• Positive IHC detected in: human skin cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67753-1-Ig targets VASH2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: VASH2 fusion protein Ag29985
• Full Name: vasohibin 2
• Calculated Molecular Weight: 355 aa, 40 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC028194
• Gene Symbol: VASH2
• Gene ID (NCBI): 79805
• RRID: AB_2918521
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VASH2, also named as vasohibin 2, is an important pro-angiogenesis factor in solid tumor. It has been reported that VASH2 is expressed in mononuclear cells mobilized from bone marrow to promote angiogenesis. VASH2 also plays a key role in axon formation. VASH2 is localized as a nuclear type(with 311 amino acid residues) and cytoplasmic type (with 355 amino acid residues and low abundance). Cytoplasmic VASH2 is associated with carcinoma angiogenesis, while nuclear VASH2 may be associated with cell proliferation. 67753-1-Ig antibody recognizes both the nuclear and cytoplasmic isoforms. (PMID:23615928; 19204325; 31235911; 26177649)