Filter: AllWBIHC

WB analysis using 67845-1-Ig (same clone as 67845-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67845-1-Ig (VAPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 67845-1-Ig (VAPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67845-1-Ig (same clone as 67845-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67845-1-Ig (VAPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67845-1-Ig (same clone as 67845-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67845-1-Ig (VAPA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67845-1-Ig (same clone as 67845-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67845-1-Ig (VAPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67845-1-Ig (same clone as 67845-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67845-1-Ig (VAPA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.

VAPA Monoclonal antibody, PBS Only

VAPA Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 67845-1-PBS

Clone No.1F10F9

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Synonyms

33 kDa VAMP associated protein, hVAP 33, VAMP A, VAMP associated protein A, VAP 33, VAP A, VAP33, VAPA

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67845-1-PBS targets VAPA in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity	Human, Mouse, Rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	VAPA fusion protein Ag7819 Predict reactive species
Full Name	VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
Calculated Molecular Weight	28 kDa
Observed Molecular Weight	33 kDa
GenBank Accession Number	BC002992
Gene Symbol	VAPA
Gene ID (NCBI)	9218
RRID	AB_2918607
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q9P0L0
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

VAPA (Vesicle-associated membrane protein-associated protein A), also known as VAP33, is a 33-kDa, ubiquitously expressed integral membrane protein of the VAMP-associated protein (VAP) family. It is present in the plasma membrane and in intracellular vesicles, playing a role in vesicle trafficking.