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VAMP3/Cellubrevin Polyclonal antibody

VAMP3/Cellubrevin Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, Blocking, ELISA

Conjugate

Unconjugated

Cat no : 10702-1-AP

Synonyms

CEB, Cellubrevin, SYB3, Synaptobrevin 3, VAMP 3, VAMP3



Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, mouse brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10702-1-AP targets VAMP3/Cellubrevin in WB, IP, IHC, IF, Blocking, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VAMP3/Cellubrevin fusion protein Ag1156
Full Name vesicle-associated membrane protein 3 (cellubrevin)
Calculated molecular weight 11 kDa
Observed molecular weight 11-17 kDa
GenBank accession numberBC005941
Gene symbol VAMP3
Gene ID (NCBI) 9341
RRIDAB_2212628
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VAMP3, also named as cellubrevin or synaptobrevin 3, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP3 is a v-SNARE (soluble NSF-attachment protein receptor). Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP3 resides in recycling endosomes and endosome-derived transport vesicles, involved in regulating membrane traffic. It is implicated in recycling of transferrin receptors, secretion of alpha-granules in platelets, and membrane trafficking during cell migration.

Protocols

Product Specific Protocols
WB protocol for VAMP3/Cellubrevin antibody 10702-1-APDownload protocol
IHC protocol for VAMP3/Cellubrevin antibody 10702-1-APDownload protocol
IP protocol for VAMP3/Cellubrevin antibody 10702-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseBlocking

Adv Sci (Weinh)

Engineered Cell-Derived Microparticles Bi2Se3/DOX@MPs for Imaging Guided Synergistic Photothermal/Low-Dose Chemotherapy of Cancer.

Authors - Dongdong Wang
humanWB,IF

Adv Sci (Weinh)

Early Endosomes Act as Local Exocytosis Hubs to Repair Endothelial Membrane Damage

Authors - Nikita Raj
humanWB,IF

Nat Commun

KRAS regulation by small non-coding RNAs and SNARE proteins.

Authors - Yonglu Che
ratIF, WB

Aging Cell

Knockdown of astrocytic Grin2a aggravates β-amyloid-induced memory and cognitive deficits through regulating nerve growth factor.

Authors - Zunshu Du
humanIHC

Oncotarget

A novel liver metastasis-correlated protein of pancreatic neuroendocrine neoplasm (PanNEN) discovered by proteomic analysis.

Authors - Mitsuhiro Shimura
mouseIF

Front Cell Dev Biol

VAMP3 and SNAP23 as Potential Targets for Preventing the Disturbed Flow-Accelerated Thrombus Formation.

Authors - Juan-Juan Zhu