Uteroglobin/CC10 Polyclonal antibody

Uteroglobin/CC10 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 26909-1-AP

Synonyms

SCGB1A1, Uteroglobin, Club cells 10 kDa secretory protein, Club cell phospholipid-binding protein, CCSP

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Validation Data Gallery

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  WB analysis of rat lung using 26909-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 26909-1-AP (Uteroglobin Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung using 26909-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 26909-1-AP (Uteroglobin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 26909-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 26909-1-AP (Uteroglobin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 26909-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26909-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 26909-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26909-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 26909-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 26909-1-AP (Uteroglobin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of A549 using 26909-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human Uteroglobin/CC10 (26909-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: rat lung tissue
• Positive IHC detected in: human lung tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Product Information

26909-1-AP targets Uteroglobin/CC10 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Uteroglobin/CC10 fusion protein Ag25530
• Full Name: secretoglobin, family 1A, member 1 (uteroglobin)
• Calculated Molecular Weight: 10 kDa
• Observed Molecular Weight: 9 kDa
• GenBank Accession Number: BC004481
• Gene Symbol: Uteroglobin
• Gene ID (NCBI): 7356
• RRID: AB_2880680
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Uteroglobin, also known as CC10, is a member of the secretoglobin family of small secreted proteins. It is produced by the nonciliated, nonmucous, secretory epithelial clara cells of the pulmonary airways, was first identified in lung lavage by Singh and colleagues. It has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma.

Publications

Species	Application	Title
mouse	IF	J Immunol; Regulation of MHC Class I Expression in Lung Epithelial Cells during Inflammation.; Authors - Justine Mathé
rat	IHC	Front Pediatr; Impaired FGF10 Signaling and Epithelial Development in Experimental Lung Hypoplasia With Esophageal Atresia.; Authors - Jun Wang
human	FC	Front Cell Infect Microbiol; BPIFA1 is a secreted biomarker of differentiating human airway epithelium; Authors - Clarissa Clifton