USP36 Recombinant antibody, PBS Only (Capture)

USP36 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241962H7

Cat no : 84620-3-PBS

Synonyms

Ubiquitin carboxyl-terminal hydrolase 36, KIAA1453, EC:2.3.2.-, DUB1, Deubiquitinating enzyme 36

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Validation Data Gallery

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  WB analysis using 84620-3-RR (same clone as 84620-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84620-3-RR (USP36 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84620-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01428-1

  Sandwich ELISA standard curve of MP01428-1, Human USP36 Recombinant Matched Antibody Pair - PBS only. 84620-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6411. 84620-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

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  Sample test of MP01428-1

  The mean USP36 concentration was determined to be 4,390.7 pg/mL in HeLa cell extract based on a 1.3 mg/mL extract load, 16,332.6 pg/mL in Jurkat cell extract based on a 1.5 mg/mL extract load and 3,553.0 pg/mL in HepG2 cell extract based on a 1.0 mg/mL extract load.

• 

  Cytometric bead array standard curve of MP01428-1

  Cytometric bead array standard curve of MP01428-1, USP36 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84620-3-PBS. Detection antibody: 84620-1-PBS. Standard: Ag6411. Range: 0.156-20 ng/mL.

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  Cytometric bead array standard curve of MP01428-2

  Cytometric bead array standard curve of MP01428-2, USP36 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84620-3-PBS. Detection antibody: 84620-2-PBS. Standard: Ag6411. Range: 0.156-20 ng/mL.

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  Affinity and Kinetic Characterization of 84620-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84620-3-RR against Human USP36 were performed. The affinity constant is 3.71 nM.

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Product Information

84620-3-PBS targets USP36 as part of a matched antibody pair:

MP01428-1: 84620-3-PBS capture and 84620-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01428-2: 84620-3-PBS capture and 84620-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: USP36 fusion protein Ag6411
• Full Name: ubiquitin specific peptidase 36
• Calculated Molecular Weight: 1123 aa, 123 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC038983
• Gene Symbol: USP36
• Gene ID (NCBI): 57602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

USP36(Ubiquitin carboxyl-terminal hydrolase 36) is also named as KIAA1453, FLJ12851, DUB1 and belongs to the peptidase C19 family. It regulates the transcription and processing of rRNA by deubiquitylating various proteins involved in these processes, and the aberrant nucleolar structure in its absence is secondary to deficient ribosome biogenesis. USP36 plays a crucial role in regulating the structure and function of nucleoli.(PMID:19208757). The ubiquitin-specific protease that is required in germline, epithelial, and intestinal stem cells(PMID:19039105). It has 2 isoforms produced by alternative splicing.