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  • KD/KO Validated

USP36 Polyclonal antibody

USP36 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 14783-1-AP


Deubiquitinating enzyme 36, DUB1, KIAA1453, Ubiquitin thiolesterase 36, USP36

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells
Positive IP detected inJurkat cells
Positive IF detected inU2OS cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14783-1-AP targets USP36 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP36 fusion protein Ag6411
Full Name ubiquitin specific peptidase 36
Calculated Molecular Weight 1123 aa, 123 kDa
Observed Molecular Weight 100-150 kDa
GenBank Accession NumberBC038983
Gene Symbol USP36
Gene ID (NCBI) 57602
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP36(Ubiquitin carboxyl-terminal hydrolase 36) is also named as KIAA1453, FLJ12851, DUB1 and belongs to the peptidase C19 family. It regulates the transcription and processing of rRNA by deubiquitylating various proteins involved in these processes, and the aberrant nucleolar structure in its absence is secondary to deficient ribosome biogenesis. USP36 plays a crucial role in regulating the structure and function of nucleoli.(PMID:19208757). The ubiquitin-specific protease that is required in germline, epithelial, and intestinal stem cells(PMID:19039105). It has 2 isoforms produced by alternative splicing.


Product Specific Protocols
WB protocol for USP36 antibody 14783-1-APDownload protocol
IF protocol for USP36 antibody 14783-1-APDownload protocol
IP protocol for USP36 antibody 14783-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Med

Thymosin α1 represents a potential potent single-molecule-based therapy for cystic fibrosis.

Authors - Luigina Romani

Nat Commun

USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress

Authors - Kewei Qin
  • KD Validated

Nucleic Acids Res

The deubiquitinase USP36 Regulates DNA replication stress and confers therapeutic resistance through PrimPol stabilization.

Authors - Yuanliang Yan
  • KD Validated

Cell Death Differ

Prostate-specific oncogene OTUD6A promotes prostatic tumorigenesis via deubiquitinating and stabilizing c-Myc.

Authors - Yunhua Peng
  • KO Validated

Int J Mol Sci

Anakinra Activates Superoxide Dismutase 2 to Mitigate Inflammasome Activity.

Authors - Marilena Pariano
humanWB, IHC, IF

Front Cell Dev Biol

USP36-Mediated Deubiquitination of DOCK4 Contributes to the Diabetic Renal Tubular Epithelial Cell Injury via Wnt/β-Catenin Signaling Pathway.

Authors - Suwei Zhu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Georgia (Verified Customer) (02-06-2023)

worked nicely in IF worked nice in WB, gave an extra band in lower molecular weight

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: WB. 1:1000 in TBS-5% milk, and IF 1:500
  • Cell Tissue Type: HeLa