Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, HepG2 cells |
| Positive IP detected in | Jurkat cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14783-1-AP targets USP36 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6411 Product name: Recombinant human USP36 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-285 aa of BC038983 Sequence: MPIVDKLKEALKPGRKDSADDGELGKLLASSAKKVLLQKIEFEPASKSFSYQLEALKSKYVLLNPKTEGASRHKSGDDPPARRQGSEHTYESCGDGVPAPQKVLFPTERLSLRWERVFRVGAGLHNLGNTCFLNATIQCLTYTPPLANYLLSKEHARSCHQGSFCMLCVMQNHIVQAFANSGNAIKPVSFIRDLKKIARHFRFGNQEDAHEFLRYTIDAMQKACLNGCAKCVLPHPPSQGFGHLFYMVAAEHSYGNSQPGTFLPRLPLGRRGRICLWVLKLLEPK Predict reactive species |
| Full Name | ubiquitin specific peptidase 36 |
| Calculated Molecular Weight | 1123 aa, 123 kDa |
| Observed Molecular Weight | 100-150 kDa |
| GenBank Accession Number | BC038983 |
| Gene Symbol | USP36 |
| Gene ID (NCBI) | 57602 |
| RRID | AB_2213357 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P275 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
USP36(Ubiquitin carboxyl-terminal hydrolase 36) is also named as KIAA1453, FLJ12851, DUB1 and belongs to the peptidase C19 family. It regulates the transcription and processing of rRNA by deubiquitylating various proteins involved in these processes, and the aberrant nucleolar structure in its absence is secondary to deficient ribosome biogenesis. USP36 plays a crucial role in regulating the structure and function of nucleoli.(PMID:19208757). The ubiquitin-specific protease that is required in germline, epithelial, and intestinal stem cells(PMID:19039105). It has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for USP36 antibody 14783-1-AP | Download protocol |
| IP protocol for USP36 antibody 14783-1-AP | Download protocol |
| WB protocol for USP36 antibody 14783-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
USP36 antibody (Cat# 14783-1-AP) has 21 citations in journals including Nature Medicine, Nature Communications, Nucleic Acids Research, Cell Death & Differentiation, Oncogene, JBC, Cancer Research Communications, and FEBS Letters. Research fields cover cancer biology (glioblastoma, prostate, breast, colorectal, lung, esophageal cancers), ribosome biogenesis, RNA processing, ferroptosis, NASH-to-HCC progression, diabetic kidney injury, cystic fibrosis, and osteoblast differentiation.
| Species | Application | Title |
|---|---|---|
Nat Med Thymosin α1 represents a potential potent single-molecule-based therapy for cystic fibrosis. | ||
Nat Commun USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress
| ||
Nat Commun Intracellular energy controls dynamics of stress-induced ribonucleoprotein granules | ||
Nucleic Acids Res The ubiquitin-specific protease USP36 SUMOylates EXOSC10 and promotes the nucleolar RNA exosome function in rRNA processing | ||
Nucleic Acids Res The deubiquitinase USP36 Regulates DNA replication stress and confers therapeutic resistance through PrimPol stabilization.
| ||
Cell Death Differ Prostate-specific oncogene OTUD6A promotes prostatic tumorigenesis via deubiquitinating and stabilizing c-Myc.
|
Reviews
What customers say
The user rated the antibody 4 out of 5 stars. They tested it on HeLa cells for both Western Blot (1:1000 in TBS-5% milk) and Immunofluorescence (1:500). The antibody worked nicely in IF and performed well in WB, though it produced an extra band at a lower molecular weight. Overall, the reviewer found the product reliable for both applications with only a minor concern about an additional non-specific band in WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Georgia (Verified Customer) (02-06-2023) | worked nicely in IF worked nice in WB, gave an extra band in lower molecular weight
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