USP10 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP10 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

12

Reviews (1)

Cat no : 19374-1-AP

Synonyms

Deubiquitinating enzyme 10, KIAA0190, Ubiquitin thiolesterase 10, UBPO, USP10

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Validation Data Gallery

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  WB analysis using 19374-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19374-1-AP (USP10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19374-1-AP

  IP result of anti-USP10 (IP:19374-1-AP, 4ug; Detection:19374-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IF Staining of HEK-293 using 19374-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using USP10 antibody (19374-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 19374-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using USP10 antibody (19374-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HEK-293 cells
• Positive IP detected in: HeLa cells
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 19374-1-AP is USP10 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP10 fusion protein Ag7233
• Full Name: ubiquitin specific peptidase 10
• Calculated Molecular Weight: 87 kDa, 92 kDa
• Observed Molecular Weight: 100-130 kDa
• GenBank Accession Number: BC000263
• Gene Symbol: USP10
• Gene ID (NCBI): 9100
• RRID: AB_10858617
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP10, also named as USP32 and NY-REN-60, belongs to the peptidase C19 family.The deubiquitinating protease USP10 has been identified as a new regulator of p53 in the DNA damage response and tumor development.

Publications

Species	Application	Title
human		Cell; G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.; Authors - Peiguo Yang
	WB	Mol Cell; Translational Repression of G3BP in Cancer and Germ Cells Suppresses Stress Granules and Enhances Stress Tolerance.; Authors - Anna K Lee
human	WB,IHC,IF,IP	Cell Death Differ; Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma; Authors - Yu-Fei Cao ; KD Validated
human	WB	Nat Commun; Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.; Authors - Hu Lei ; KD Validated
human	IF	J Cell Biol; High-fidelity reconstitution of stress granules and nucleoli in mammalian cellular lysate.; Authors - Brian D Freibaum
mouse	WB	Br J Pharmacol; Limonin stabilises SIRT6 by activating USP10 in cardiac hypertrophy.; Authors - Li-Bo Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Gonzalo (Verified Customer) (03-21-2018)

Great antibody for WB and IP

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293

USP10 Antibody Western Blot,Immunoprecipitation, validation (1:1000 dilution) in HEK293 (Cat no:19374-1-AP)