USF2 Polyclonal antibody

• Featured Product
• KD/KO Validated

USF2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16614-1-AP

Synonyms

bHLHb12, FIP, FOS interacting protein, Upstream stimulatory factor 2, USF2

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Validation Data Gallery

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  WB analysis of Jurkat using 16614-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16614-1-AP (USF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16614-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16614-1-AP (USF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

16614-1-AP targets USF2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USF2 fusion protein Ag9866
• Full Name: upstream transcription factor 2, c-fos interacting
• Calculated Molecular Weight: 346 aa, 37 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC049821
• Gene Symbol: USF2
• Gene ID (NCBI): 7392
• RRID: AB_1851709
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB	Ann Hematol; USF2 promotes autophagy and proliferation in chronic lymphocytic leukemia by inhibiting STUB1-induced NFAT5 ubiquitination; Authors - Beili Chen ; KD Validated
	WB,IHC	Dev Genes Evol; A novel protein upstream stimulatory factor 2 identified in lamprey, Lethenteron reissneri.; Authors - Yuxuan Guo