USF2 Polyclonal antibody

• 

  WB analysis of Jurkat using 16614-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16614-1-AP (USF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16614-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16614-1-AP (USF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

USF2 Polyclonal Antibody for WB, ELISA

Cat No. 16614-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

bHLHb12, FIP, FOS interacting protein, Upstream stimulatory factor 2, USF2

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Tested Applications

• Positive WB detected in: Jurkat cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

16614-1-AP targets USF2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9866

  Product name: Recombinant human USF2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-346 aa of BC049821

  Sequence: MDMLDPGLDPAASATAAAAASHDKGPEAEEGVELQEGGDGPGAEEQTAVAITSVQQAAFGDHNIQYQFRTETNGGQVTYRVVQVTDGQLDGQGDTAGAVSVVSTAAFAGGQQAVTQVGVDGAAQRPGPAAASVPPGPAAPFPLAVIQNPFSNGGSPAAEAVSGEARFAYFPASSVGDTTAVSVQTTDQSLQAGGQFYVMMTPQDVLQTGTQRTIAPRTHPYSPKIDGTRTPRDERRRAQHNEVERRRRDKINNWIVQLSKIIPDCNADNSKTGASKGGILSKACDYIRELRQTNQRMQETFKEAERLQMDNELLRQQIEELKNENALLRAQLQQHNLEMVGEGTRQ

• Full Name: upstream transcription factor 2, c-fos interacting
• Calculated Molecular Weight: 346 aa, 37 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC049821
• Gene Symbol: USF2
• Gene ID (NCBI): 7392
• RRID: AB_1851709
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15853
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	WB	Ann Hematol; USF2 promotes autophagy and proliferation in chronic lymphocytic leukemia by inhibiting STUB1-induced NFAT5 ubiquitination; Authors - Beili Chen ; KD Validated
human	WB	Int Heart J; Upstream Stimulatory Factor 2 Protects Cardiomyocytes by Regulating Mitochondrial Homeostasis; Authors - Wenbin Wu ; KD Validated
	WB,IHC	Dev Genes Evol; A novel protein upstream stimulatory factor 2 identified in lamprey, Lethenteron reissneri.; Authors - Yuxuan Guo
human	WB	Oncogene; YBX1 promotes 5-Fluorouracil resistance in gastric cancer via m5C-dependent ATG9A mRNA stabilization through autophagy; Authors - Hongxin Huang ; KD Validated