UROC1 Polyclonal antibody

UROC1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25032-1-AP

Synonyms

HMFN0320, UROC1, Urocanase, urocanase domain containing 1, Urocanate hydratase

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Validation Data Gallery

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  WB analysis of mouse liver using 25032-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25032-1-AP (UROC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 25032-1-AP

  IP result of anti-UROC1 (IP:25032-1-AP, 4ug; Detection:25032-1-AP 1:300) with mouse liver tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IP detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

25032-1-AP targets UROC1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UROC1 fusion protein Ag18143
• Full Name: urocanase domain containing 1
• Calculated Molecular Weight: 676 aa, 75 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC115405
• Gene Symbol: UROC1
• Gene ID (NCBI): 131669
• RRID: AB_2879859
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UROC1, also named as Urocanase and Imidazolonepropionate hydrolase, is involved in Urocanase deficiency (UROCD). UROC1 has some isoforms with MW 65-75 kDa and 80 kDa.

Publications

Species	Application	Title
mouse	WB	EMBO Mol Med; Luteolin detoxifies DEHP and prevents liver injury by degrading Uroc1 protein in mice; Authors - Huiting Wang