UGT3A2 Polyclonal antibody

UGT3A2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 21366-1-AP

Synonyms

UDPGT 3A2, UGT3A2, UNQ842/PRO1780

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Validation Data Gallery

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  WB analysis using 21366-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21366-1-AP (UGT3A2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 21366-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21366-1-AP (UGT3A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 21366-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 21366-1-AP (UGT3A2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 21366-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 21366-1-AP (UGT3A2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MOLT-4 cells, human testis tissue, THP-1 cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

The immunogen of 21366-1-AP is UGT3A2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UGT3A2 fusion protein Ag15835
• Full Name: UDP glycosyltransferase 3 family, polypeptide A2
• Calculated Molecular Weight: 523 aa, 60 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC103925
• Gene Symbol: UGT3A2
• Gene ID (NCBI): 167127
• RRID: AB_10888629
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
	IHC	Front Mol Biosci; Identification of small extracellular vesicle protein biomarkers for pediatric Ewing Sarcoma; Authors - Soumya M Turaga

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Daniel (Verified Customer) (10-26-2022)

This antibody works but produces high background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa, MT4 cells

UGT3A2 Antibody Western Blot validation (1:500 dilution) in HeLa, MT4 cells (Cat no:21366-1-AP)