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UCMA Polyclonal antibody

UCMA Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25503-1-AP

Synonyms

C10orf49, Gla rich protein, GRP, UCMA, Ucma C

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using UCMA Polyclonal antibody (25503-1-AP)

    WB analysis of mouse spleen using 25503-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25503-1-AP (UCMA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25503-1-AP is UCMA Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UCMA fusion protein Ag21985
Full Name upper zone of growth plate and cartilage matrix associated
Calculated Molecular Weight 138 aa, 17 kDa
Observed Molecular Weight ~14 kDa
GenBank Accession NumberBC018068
Gene Symbol UCMA
Gene ID (NCBI) 221044
RRIDAB_2880106
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UCMA, also named as C10orf49, Ucma-C and GRP, is a 17 kDa small, highly charged, and secreted protein. It has been described as a novel secretory protein mainly expressed in cartilage and also as a novel vitamin-K-dependent protein. UCMA is involved in the negative control of osteogenic differentiation of osteochondrogenic precursor cells in peripheral zones of fetal cartilage and at the cartilage-bone interface.

Protocols

Product Specific Protocols
WB protocol for UCMA antibody 25503-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols