Tested Applications
| Positive WB detected in | HEK-293 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21052-1-AP targets UBXD2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13440 Product name: Recombinant human UBXN4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 160-508 aa of BC035594 Sequence: TSDTKSDTATGGESAGHATSSQEPSGCSDQRPAEDLNIRVERLTKKLEERREEKRKEEEQREIKKEIERRKTGKEMLDYKRKQEEELTKRMLEERNREKAEDRAARERIKQQIALDRAERAARFAKTKEEVEAAKAAALLAKQAEMEVKRESYARERSTVARIQFRLPDGSSFTNQFPSDAPLEEARQFAAQTVGNTYGNFSLATMFPRREFTKEDYKKKLLDLELAPSASVVLLPAGRPTASIVHSSSGDIWTLLGTVLYPFLAIWRLISNFLFSNPPPTQTSVRVTSSEPPNPASSSKSEKREPVRKRVLEKRGDDFKKEGKIYRLRTQDDGEDENNTWNGNSTQQM Predict reactive species |
| Full Name | UBX domain protein 4 |
| Calculated Molecular Weight | 508 aa, 57 kDa |
| Observed Molecular Weight | 64-68 kDa |
| GenBank Accession Number | BC035594 |
| Gene Symbol | UBXD2 |
| Gene ID (NCBI) | 23190 |
| RRID | AB_10732809 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92575 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for UBXD2 antibody 21052-1-AP | Download protocol |
| IHC protocol for UBXD2 antibody 21052-1-AP | Download protocol |
| IP protocol for UBXD2 antibody 21052-1-AP | Download protocol |
| WB protocol for UBXD2 antibody 21052-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The UBXD2 (UBXN4) polyclonal antibody (Cat# 21052-1-AP) has 7 total citations published in Nature Communications, Journal of Clinical Investigation, Apoptosis, Journal of Cell Biology, EMBO Reports, Journal of Cell Science, and bioRxiv. Research fields include ER–mitochondria contact site regulation, ubiquitination–proteasome coupling in ischemia-reperfusion injury, hypoxia/reoxygenation-induced apoptosis and autophagy, peroxisome maintenance and pexophagy suppression, mitochondria-associated degradation restraining apoptosis and mitophagy, and aggresome formation.
| Species | Application | Title |
|---|---|---|
Nat Commun The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition | ||
J Clin Invest Inadequate ubiquitination-proteasome coupling contributes to myocardial ischemia-reperfusion injury. | ||
Apoptosis Acetylcholine exerts cytoprotection against hypoxia/reoxygenation-induced apoptosis, autophagy and mitochondrial impairment through both muscarinic and nicotinic receptors. | ||
J Cell Biol The p97 ATPase and its adaptor UBXD8 maintain peroxisome pools by preventing pexophagy. | ||
EMBO Rep UBXD8 mediates mitochondria-associated degradation to restrain apoptosis and mitophagy | ||
