UBXD2 Polyclonal antibody

UBXD2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 21052-1-AP

Synonyms

erasin, KIAA0242, KIAA2042, UBX domain protein 4, UBXD2, UBXDC1, UBXN4

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Validation Data Gallery

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  WB analysis using 21052-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21052-1-AP (UBXD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21052-1-AP

  IP result of anti-UBXD2 (IP:21052-1-AP, 4ug; Detection:21052-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human colon cancer using 21052-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21052-1-AP (UBXD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 21052-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using UBXD2 antibody (21052-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 21052-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using UBXD2 antibody (21052-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 21052-1-AP is UBXD2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBXD2 fusion protein Ag13440
• Full Name: UBX domain protein 4
• Calculated Molecular Weight: 508 aa, 57 kDa
• Observed Molecular Weight: 64-68 kDa
• GenBank Accession Number: BC035594
• Gene Symbol: UBXD2
• Gene ID (NCBI): 23190
• RRID: AB_10732809
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	CoIP	J Clin Invest; Inadequate ubiquitination-proteasome coupling contributes to myocardial ischemia-reperfusion injury.; Authors - Chengjun Hu
human	IF	J Cell Sci; The p97-UBXN1 complex regulates aggresome formation.; Authors - Sirisha Mukkavalli
rat	WB	Apoptosis; Acetylcholine exerts cytoprotection against hypoxia/reoxygenation-induced apoptosis, autophagy and mitochondrial impairment through both muscarinic and nicotinic receptors.; Authors - Kannaporn Intachai
human	WB,IF	EMBO Rep; UBXD8 mediates mitochondria-associated degradation to restrain apoptosis and mitophagy; Authors - Jing Zheng
human	WB	Nat Commun; The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition; Authors - Rakesh Ganji