UBXN1 Polyclonal antibody

• Featured Product
• KD/KO Validated

UBXN1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 16135-1-AP

Synonyms

2B28, LOC51035, SAKS1, SAPK substrate protein 1, UBA/UBX 33.3 kDa protein, UBX domain protein 1, UBXD10, UBXN1

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Validation Data Gallery

• 

  WB analysis using 16135-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16135-1-AP (UBXN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16135-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16135-1-AP (UBXN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 16135-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 16135-1-AP (UBXN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16135-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16135-1-AP (UBXN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 16135-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16135-1-AP (UBXN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 16135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16135-1-AP (UBXN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 16135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16135-1-AP (UBXN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, human liver tissue, mouse cerebellum tissue, human brain tissue, human heart tissue, HepG2 cells, Ramos cells, SH-SY5Y cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:30000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Product Information

16135-1-AP targets UBXN1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBXN1 fusion protein Ag9146
• Full Name: UBX domain protein 1
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC000902
• Gene Symbol: UBXN1
• Gene ID (NCBI): 51035
• RRID: AB_2210480
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	EMBO J; A weakened interface in the P182L variant of HSP27 associated with severe Charcot-Marie-Tooth neuropathy causes aberrant binding to interacting proteins.; Authors - T Reid Alderson
human	WB	Cancer Lett; The CRISPR/Cas9 system targeting EGFR exon 17 abrogates NF-κB activation via epigenetic modulation of UBXN1 in EGFRwt/vIII glioma cells.; Authors - Kai Huang
human,mouse	WB,IHC	Front Immunol; PTRF/Cavin-1 as a Novel RNA-Binding Protein Expedites the NF-κB/PD-L1 Axis by Stabilizing lncRNA NEAT1, Contributing to Tumorigenesis and Immune Evasion in Glioblastoma.; Authors - Kaikai Yi
human	WB	Sci Rep; PMID: 29769705; Authors - Shotaro Uchida
human	WB,IF	J Cell Sci; The p97-UBXN1 complex regulates aggresome formation.; Authors - Sirisha Mukkavalli ; KO Validated
human	WB,IP,CoIP,IF	Mol Cell Biol; The VCP-UBXN1 complex mediates triage of ubiquitylated cytosolic proteins bound to the BAG6 complex.; Authors - Rakesh Ganji ; KD Validated