Trappc9,NIBP Polyclonal antibody

Trappc9,NIBP Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20176-1-AP

Synonyms

1810044A24Rik, 2900005P22Rik, 4632408O18Rik, Ibp, KIAA1882, mKIAA1882, NIBP, Trappc9, TRS130

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Validation Data Gallery

• 

  WB analysis of Neuro-2a using 20176-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 20176-1-AP (Trappc9,NIBP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 20176-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20176-1-AP (Trappc9,NIBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20176-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20176-1-AP (Trappc9,NIBP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 20176-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20176-1-AP (Trappc9,NIBP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Neuro-2a cells, mouse brain tissue, mouse skeletal muscle tissue, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20176-1-AP is Trappc9,NIBP Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Trappc9,NIBP fusion protein Ag14018
• Full Name: trafficking protein particle complex 9
• Calculated Molecular Weight: 129/250/128 kDa
• Observed Molecular Weight: 128 kDa, 139 kDa
• GenBank Accession Number: BC070463
• Gene Symbol: NIBP/Trappc9
• Gene ID (NCBI): 76510
• RRID: AB_10694434
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAPPC9, also named as KIAA1882 and NIBP, belongs to the NIBP family. It functions as an activator of NF-kappa-B through increased phosphorylation of the IKK complex. TRAPPC9 may function in neuronal cells differentiation. It may play a role in vesicular transport from endoplasmic reticulum to Golgi. TRAPPC9 was found in neurons of the cerebral cortex, hippocampus, and deep gray matter. In WB, the MW of TRAPPC9 is 130-140kd. A 250kd maybe the TRAPPC9 undergoes extensive posttranslational modification.