Transgelin-2-specific Polyclonal antibody

• Featured Product
• KD/KO Validated

Transgelin-2-specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 15508-1-AP

Synonyms

Transgelin 2, TAGLN2, Transgelin 2/TAGLN2, SM22-alpha homolog, Epididymis tissue protein Li 7e

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Validation Data Gallery

• 

  WB analysis of HeLa using 15508-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15508-1-AP (Transgelin-2-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15508-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15508-1-AP (Transgelin-2-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 15508-1-AP

  IP result of anti-Transgelin-2-specific (IP:15508-1-AP, 3ug; Detection:15508-1-AP 1:500) with L02 cells lysate 1000ug.

• 

  IHC staining of human kidney using 15508-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15508-1-AP (Transgelin-2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human oesophagus cancer using 15508-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15508-1-AP (Transgelin-2-specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 15508-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15508-1-AP (Transgelin-2-specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15508-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15508-1-AP (Transgelin-2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human normal colon using 15508-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15508-1-AP (Transgelin-2-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 15508-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 15508-1-AP (Transgelin-2-specific Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 15508-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 15508-1-AP (Transgelin-2-specific Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 15508-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Transgelin-2-specific antibody (15508-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive IP detected in: L02 cells
• Positive IHC detected in: human oesophagus cancer tissue, human kidney tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

15508-1-AP targets Transgelin-2-specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: transgelin 2
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: NM_003564
• Gene Symbol: Transgelin-2/TAGLN2
• Gene ID (NCBI): 8407
• RRID: AB_2199501
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody can bind transgelin 2 specifically, but not trangelin 1 or 3.

Publications

Species	Application	Title
human	WB	Front Oncol; An Essential NRP1-Mediated Role for Tagln2 in Gastric Cancer Angiogenesis.; Authors - Hongwei Jin ; KD Validated
human	WB, IHC	J Gastroenterol Hepatol; The tumor-suppressive function of miR-1 by targeting LASP1 and TAGLN2 in esophageal squamous cell carcinoma.; Authors - Yan-Yan Du
human	WB	Cancer Cell Int; MiR-613 inhibits the proliferation, migration, and invasion of papillary thyroid carcinoma cells by directly targeting TAGLN2.; Authors - Yonglian Huang
human	WB,IF	PLoS One; Transgelin 2 participates in lovastatin-induced anti-angiogenic effects in endothelial cells through a phosphorylated myosin light chain-related mechanism.; Authors - Xiao Yuan Y ; KD Validated
human	WB,IP	Biochem Biophys Res Commun; Transgelin-2 contributes to proliferation and progression of hepatocellular carcinoma via regulating Annexin A2.; Authors - Jingpei Shi
human	WB	Molecules; MOBT Alleviates Pulmonary Fibrosis through an lncITPF-hnRNP-l-Complex-Mediated Signaling Pathway; Authors - Pan Xu

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