Gamma Tubulin Polyclonal antibody
Gamma Tubulin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, Canine
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 15176-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, Transfected HEK-293 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human prostate cancer tissue, human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | hTERT-RPE cells , MDCK cells, HUVEC cells, hTERT-RPE cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 3 publications below |
IHC | See 1 publications below |
IF | See 7 publications below |
Product Information
15176-1-AP targets Gamma Tubulin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.
Tested Reactivity | human, mouse, rat, Canine |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Gamma Tubulin fusion protein Ag7070 |
Full Name | tubulin, gamma 1 |
Calculated molecular weight | 51 kDa |
Observed molecular weight | 50-55 kDa |
GenBank accession number | BC000619 |
Gene symbol | TUBG1 |
Gene ID (NCBI) | 7283 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.
Protocols
Product Specific Protocols | |
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WB protocol for Gamma Tubulin antibody 15176-1-AP | Download protocol |
IHC protocol for Gamma Tubulin antibody 15176-1-AP | Download protocol |
IF protocol for Gamma Tubulin antibody 15176-1-AP | Download protocol |
IP protocol for Gamma Tubulin antibody 15176-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Hum Mol Genet Nearly complete deletion of BubR1 causes microcephaly through shortened mitosis and massive cell death. | ||
FEBS Open Bio Down-regulation of Polo-like kinase 4 (PLK4) induces G1 arrest via activation of the p38/p53/p21 signaling pathway in bladder cancer. | ||
Sci Rep Centrosome and ciliary abnormalities in fetal akinesia deformation sequence human fibroblasts. | ||
J Cell Mol Med Whole-exome sequencing identified novel variants in CPLANE1 that causes oral-facial-digital syndrome Ⅵ by inducing primary cilia abnormality. | ||
J Cell Sci Retromer/WASH dependent sorting of nutrient transporters requires a multivalent interaction network with ANKRD50. | ||
Front Cell Dev Biol LUZP1 Controls Cell Division, Migration and Invasion Through Regulation of the Actin Cytoskeleton. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Iru (Verified Customer) (06-17-2021) | It works well with O/N incubation.
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FH Christopher (Verified Customer) (07-02-2019) | Very fast delivery I think it arrived in a matter of days which is quick compared to some other companies.Westerns worked really well, so it must be good, over all really pleased with this purchase
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FH Boyan (Verified Customer) (01-29-2019) | Excellent. Works quite well both for WB and IF.
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