Gamma Tubulin Polyclonal antibody

Gamma Tubulin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

11

Reviews (3)

Cat no : 15176-1-AP

Synonyms

Gamma 1 tubulin, gamma Tubulin, GCP 1, TUBG, TUBG1, TUBGCP1, Tubulin gamma 1 chain, tubulin, gamma 1

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Validation Data Gallery

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  WB analysis of HeLa using 15176-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15176-1-AP (gamma Tubulin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 15176-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 15176-1-AP (gamma Tubulin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15176-1-AP

  IP Result of anti-gamma Tubulin (IP:15176-1-AP, 4ug; Detection:15176-1-AP 1:2000) with HeLa cells lysate 1600ug.

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  IHC staining of human prostate cancer using 15176-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15176-1-AP (gamma Tubulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15176-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15176-1-AP (gamma Tubulin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate hyperplasia using 15176-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15176-1-AP (gamma Tubulin antibody at dilution of 1:400 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 15176-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15176-1-AP (gamma Tubulin antibody at dilution of 1:400 (under 40x lens).

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  IF Staining of hTERT-RPE cells using 15176-1-AP

  IF results of gamma tubulin antibody (15176-1-AP, 1:100) with hTERT-RPE cells by Moshe Kim (laboratory of Dr. William S Trimble, University of Toronto). Cells were fixed in ice-cold methanol.

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  IF Staining of hTERT-RPE cells using 15176-1-AP

  IF results of gamma tubulin antibody (15176-1-AP, 1:100) with hTERT-RPE cells by Moshe Kim (laboratory of Dr. William S Trimble, University of Toronto). Cells were fixed in ice-cold methanol.

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  IF Staining of hTERT-RPE cells using 15176-1-AP

  IF results of gamma tubulin antibody (15176-1-AP, 1:100) with hTERT-RPE cells by Moshe Kim (laboratory of Dr. William S Trimble, University of Toronto). Cells were fixed in ice-cold methanol.

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  IF Staining of MDCK using 15176-1-AP

  Immunofluorescent analysis of MDCK cells using 15176-1-AP (gamma Tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MDCK using 15176-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using Gamma Tubulin antibody (15176-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HUVEC using 15176-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using Gamma Tubulin antibody (15176-1-AP) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MDCK using 15176-1-AP

  Immunofluorescent analysis of MDCK cells using 15176-1-AP (gamma Tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MDCK using 15176-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed MDCK cells using 15176-1-AP (gamma Tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 15176-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Gamma Tubulin (15176-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, Transfected HEK-293 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue, human prostate hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: hTERT-RPE cells , MDCK cells, HUVEC cells, hTERT-RPE cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 7 publications below

Product Information

15176-1-AP targets Gamma Tubulin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

• Tested Reactivity: human, mouse, rat, Canine
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Gamma Tubulin fusion protein Ag7070
• Full Name: tubulin, gamma 1
• Calculated molecular weight: 51 kDa
• Observed molecular weight: 50-55 kDa
• GenBank accession number: BC000619
• Gene symbol: TUBG1
• Gene ID (NCBI): 7283
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.

Publications

Species	Application	Title
mouse	IF	Hum Mol Genet; Nearly complete deletion of BubR1 causes microcephaly through shortened mitosis and massive cell death.; Authors - Ambrosia J Simmons
human	IF	FEBS Open Bio; Down-regulation of Polo-like kinase 4 (PLK4) induces G1 arrest via activation of the p38/p53/p21 signaling pathway in bladder cancer.; Authors - Ziyi Yang
human	IF	Sci Rep; Centrosome and ciliary abnormalities in fetal akinesia deformation sequence human fibroblasts.; Authors - Ramona Jühlen
mouse	IF	J Cell Mol Med; Whole-exome sequencing identified novel variants in CPLANE1 that causes oral-facial-digital syndrome Ⅵ by inducing primary cilia abnormality.; Authors - Wen Qian
		J Cell Sci; Retromer/WASH dependent sorting of nutrient transporters requires a multivalent interaction network with ANKRD50.; Authors - Arunas Kvainickas
mouse	IHC	Front Cell Dev Biol; LUZP1 Controls Cell Division, Migration and Invasion Through Regulation of the Actin Cytoskeleton.; Authors - Laura Bozal-Basterra

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iru (Verified Customer) (06-17-2021)

It works well with O/N incubation.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fallopian tube epithelial cells

Gamma Tubulin Antibody Western Blot validation (1:1000 dilution) in Fallopian tube epithelial cells (Cat no:15176-1-AP)

FH

Christopher (Verified Customer) (07-02-2019)

Very fast delivery I think it arrived in a matter of days which is quick compared to some other companies.Westerns worked really well, so it must be good, over all really pleased with this purchase

• Applications: Western Blot,

FH

Boyan (Verified Customer) (01-29-2019)

Excellent. Works quite well both for WB and IF.

• Applications: Western Blot, Immunofluorescence,

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C