Gamma Tubulin Polyclonal antibody

Gamma Tubulin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15176-1-AP

Synonyms

Gamma 1 tubulin, gamma Tubulin, GCP 1, TUBG, TUBG1, TUBGCP1, Tubulin gamma 1 chain, tubulin, gamma 1



Tested Applications

Positive WB detected inHeLa cells, Transfected HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE cells , MDCK cells, HUVEC cells, hTERT-RPE cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:200
Sample-dependent, check data in validation data gallery

Product Information

15176-1-AP targets Gamma Tubulin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Reactivity human, mouse, rat, Canine
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Gamma Tubulin fusion protein Ag7070
Full Name tubulin, gamma 1
Calculated molecular weight 51 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC000619
Gene symbol TUBG1
Gene ID (NCBI) 7283
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.

Protocols

Product Specific Protocols
WB protocol for Gamma Tubulin antibody 15176-1-APDownload protocol
IHC protocol for Gamma Tubulin antibody 15176-1-APDownload protocol
IF protocol for Gamma Tubulin antibody 15176-1-APDownload protocol
IP protocol for Gamma Tubulin antibody 15176-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Hum Mol Genet

Nearly complete deletion of BubR1 causes microcephaly through shortened mitosis and massive cell death.

Authors - Ambrosia J Simmons
humanIF

FEBS Open Bio

Down-regulation of Polo-like kinase 4 (PLK4) induces G1 arrest via activation of the p38/p53/p21 signaling pathway in bladder cancer.

Authors - Ziyi Yang
humanIF

Sci Rep

Centrosome and ciliary abnormalities in fetal akinesia deformation sequence human fibroblasts.

Authors - Ramona Jühlen
mouseIF

J Cell Mol Med

Whole-exome sequencing identified novel variants in CPLANE1 that causes oral-facial-digital syndrome Ⅵ by inducing primary cilia abnormality.

Authors - Wen Qian

J Cell Sci

Retromer/WASH dependent sorting of nutrient transporters requires a multivalent interaction network with ANKRD50.

Authors - Arunas Kvainickas
mouseIHC

Front Cell Dev Biol

LUZP1 Controls Cell Division, Migration and Invasion Through Regulation of the Actin Cytoskeleton.

Authors - Laura Bozal-Basterra

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Iru (Verified Customer) (06-17-2021)

It works well with O/N incubation.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Fallopian tube epithelial cells
Gamma Tubulin Antibody Western Blot validation (1:1000 dilution) in Fallopian tube epithelial cells (Cat no:15176-1-AP)
FH

Christopher (Verified Customer) (07-02-2019)

Very fast delivery I think it arrived in a matter of days which is quick compared to some other companies.Westerns worked really well, so it must be good, over all really pleased with this purchase

  • Applications: Western Blot,
FH

Boyan (Verified Customer) (01-29-2019)

Excellent. Works quite well both for WB and IF.

  • Applications: Western Blot, Immunofluorescence,