Gamma Tubulin Polyclonal antibody

• 

  WB analysis of HeLa using 15176-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15176-1-AP (gamma Tubulin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 15176-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 15176-1-AP (gamma Tubulin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15176-1-AP

  IP result of anti-Gamma Tubulin (IP:15176-1-AP, 4ug; Detection:15176-1-AP 1:2000) with HeLa cells lysate 1600ug.

• 

  IHC staining of human prostate cancer using 15176-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15176-1-AP (gamma Tubulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15176-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15176-1-AP (gamma Tubulin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 15176-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15176-1-AP (gamma Tubulin antibody at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 15176-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15176-1-AP (gamma Tubulin antibody at dilution of 1:400 (under 40x lens).

• 

  FC experiment of HeLa using 15176-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Gamma Tubulin (15176-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

Gamma Tubulin Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Cat No. 15176-1-AP

Publications(23)

Reviews (4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Synonyms

TUBG1, Tubulin gamma 1 chain, TUBG, GCP-1, Gamma-tubulin complex component 1

---

Tested Applications

• Positive WB detected in: HeLa cells, Transfected HEK-293 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue, human prostate hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 17 publications below

Product Information

15176-1-AP targets Gamma Tubulin in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Gamma Tubulin fusion protein Ag7070
• Full Name: tubulin, gamma 1
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC000619
• Gene Symbol: Gamma Tubulin
• Gene ID (NCBI): 7283
• RRID: AB_2878113
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P23258
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.

Publications

Species	Application	Title
human	IF	Cancer Cell; Hippo Signaling Suppresses Cell Ploidy and Tumorigenesis through Skp2.; Authors - Shihao Zhang
human	IF,WB	Nat Commun; INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex; Authors - Lauren G. Mascibroda
mouse	IF	PLoS Biol; Noncoding RNA Ginir functions as an oncogene by associating with centrosomal proteins.; Authors - Suchismita Panda
mouse	WB	Cell Death Differ; Haploinsufficiency of GCP4 induces autophagy and leads to photoreceptor degeneration due to defective spindle assembly in retina.; Authors - Zhigang Li
human	IF	Br J Pharmacol; NL13, a novel curcumin analogue and polo like kinase 4 inhibitor, induces cell cycle arrest and apoptosis in prostate cancer models; Authors - Xinyi Qiao
human	WB	Elife; LUZP1, a novel regulator of primary cilia and the actin cytoskeleton, is a contributing factor in Townes-Brocks Syndrome.; Authors - Laura Bozal-Basterra

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Deng (Verified Customer) (07-22-2022)

The cells were fixed with cold methanol.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 : 500
• Cell Tissue Type: Human RPE1 cells

Gamma Tubulin Antibody Immunofluorescence validation (1 : 500 dilution) in Human RPE1 cells (Cat no:15176-1-AP)

FH

Iru (Verified Customer) (06-17-2021)

It works well with O/N incubation.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fallopian tube epithelial cells

Gamma Tubulin Antibody Western Blot validation (1:1000 dilution) in Fallopian tube epithelial cells (Cat no:15176-1-AP)

FH

Christopher (Verified Customer) (07-02-2019)

Very fast delivery I think it arrived in a matter of days which is quick compared to some other companies.Westerns worked really well, so it must be good, over all really pleased with this purchase

• Applications: Western Blot,

FH

Boyan (Verified Customer) (01-29-2019)

Excellent. Works quite well both for WB and IF.

• Applications: Western Blot, Immunofluorescence,