Beta Tubulin Polyclonal antibody

Beta Tubulin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 10063-2-AP

Synonyms

Tubulin beta-2B chain, TUBB2B

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Validation Data Gallery

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  WB analysis using 10063-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10063-2-AP (Beta Tubulin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 10063-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10063-2-AP (Beta Tubulin antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 10063-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10063-2-AP (Beta Tubulin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10063-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10063-2-AP (Beta Tubulin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 10063-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10063-2-AP (Beta Tubulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse cerebellum tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

10063-2-AP targets Beta Tubulin in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Beta Tubulin fusion protein Ag0108
• Full Name: tubulin, beta 2B
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50kda
• GenBank Accession Number: BC001352
• Gene Symbol: Beta Tubulin
• Gene ID (NCBI): 347733
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

There are five tubulins in human cells. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules.

Publications

Species	Application	Title
human	CoIP	Biol Psychiatry; Molecular networks of DYX1C1 gene show connection to neuronal migration genes and cytoskeletal proteins.; Authors - Kristiina Tammimies
mouse	WB	J Am Soc Nephrol; Tubular von Hippel-Lindau knockout protects against rhabdomyolysis-induced AKI.; Authors - Michael Fähling
human	WB	Nucleic Acids Res; Hypoxia-induced gene expression results from selective mRNA partitioning to the endoplasmic reticulum.; Authors - Jonas J Staudacher
mouse	WB	Acta Physiol (Oxf); A dual role of miR-22 in rhabdomyolysis-induced acute kidney injury.; Authors - S Mathia
		Ecotoxicol Environ Saf; Cinchophen induces RPA1 related DNA damage and apoptosis to impair ENS development of zebrafish; Authors - Jing Wang
human	WB,IP	Cancer Biol Med; IL13Rα1 prevents a castration resistant phenotype of prostate cancer by targeting hexokinase 2 for ubiquitin-mediated degradation.; Authors - Tingting Feng