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  • Featured Product
  • KD/KO Validated

TTL Recombinant antibody, PBS Only

TTL Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3P9

Cat no : 82763-5-PBS

Synonyms

Tubulin--tyrosine ligase, tubulin tyrosine ligase, Tubulin tyrosine ligase, EC:6.3.2.25, 3P9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TTL Recombinant antibody (82763-5-RR)

    WB analysis using 82763-5-RR (same clone as 82763-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82763-5-RR (TTL antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82763-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TTL Recombinant antibody (82763-5-RR)

    WB analysis of HeLa using 82763-5-RR (same clone as 82763-5-PBS)

    WB result of TTL antibody (82763-5-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTL transfected HeLa cells. This data was developed using the same antibody clone with 82763-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82763-5-RR

    Affinity and Kinetic Characterization of 82763-5-RR

    Biolayer interferometry (BLl) kinetic assays of 82763-5-RR against Human TTL were performed. The affinity constant is below 1 pM.

Product Information

82763-5-PBS targets TTL in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TTL fusion protein Ag4526
Full Name tubulin tyrosine ligase
Calculated Molecular Weight 377 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC036819
Gene Symbol TTL
Gene ID (NCBI) 150465
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.