TTL Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

TTL Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E5F8

Cat no : 66076-1-PBS

Synonyms

Tubulin--tyrosine ligase, tubulin tyrosine ligase, Tubulin tyrosine ligase, EC:6.3.2.25, 2E5F8

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Validation Data Gallery

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  WB analysis using 66076-1-Ig (same clone as 66076-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66076-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66076-1-Ig (same clone as 66076-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66076-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 66076-1-Ig (same clone as 66076-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66076-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66076-1-Ig (same clone as 66076-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66076-1-Ig(TTL antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66076-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66076-1-Ig (same clone as 66076-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66076-1-Ig(TTL antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66076-1-PBS in a different storage buffer formulation.

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Product Information

66076-1-PBS targets TTL in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TTL fusion protein Ag4708
• Full Name: tubulin tyrosine ligase
• Calculated Molecular Weight: 377 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC036819
• Gene Symbol: TTL
• Gene ID (NCBI): 150465
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.