TTL Polyclonal antibody

• Featured Product
• KD/KO Validated

TTL Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

33

Reviews (1)

Cat no : 13618-1-AP

Synonyms

Tubulin--tyrosine ligase, tubulin tyrosine ligase, Tubulin tyrosine ligase, EC:6.3.2.25

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Validation Data Gallery

• 

  WB analysis of HeLa using 13618-1-AP

  WB result of TTL antibody (13618-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTL transfected HeLa cells.

• 

  WB analysis using 13618-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13618-1-AP (TTL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 13618-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13618-1-AP (TTL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13618-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13618-1-AP (TTL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 13618-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 13618-1-AP (TTL antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 13618-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 13618-1-AP (TTL antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain, rat brain
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 28 publications below
• IF: See 6 publications below

Product Information

13618-1-AP targets TTL in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TTL fusion protein Ag4526
• Full Name: tubulin tyrosine ligase
• Calculated Molecular Weight: 377 aa, 43 kDa
• Observed Molecular Weight: 43-45 kDa
• GenBank Accession Number: BC036819
• Gene Symbol: TTL
• Gene ID (NCBI): 150465
• RRID: AB_2256858
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.

Publications

Species	Application	Title
	WB	Science; Posttranslational modification of microtubules by the MATCAP detyrosinase.; Authors - Lisa Landskron
mouse	WB	Nature; MARK4 controls ischaemic heart failure through microtubule detyrosination.; Authors - Xian Yu
human	WB	Nature; Nuclear envelope assembly defects link mitotic errors to chromothripsis.; Authors - Shiwei Liu
human	WB	Science; Vasohibins encode tubulin detyrosinating activity.; Authors - Joppe Nieuwenhuis
human	WB	Science; Mitosis. Microtubule detyrosination guides chromosomes during mitosis.; Authors - Marin Barisic
human	WB	Nat Med; Suppression of detyrosinated microtubules improves cardiomyocyte function in human heart failure.; Authors - Christina Yingxian Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

WEI (Verified Customer) (05-11-2022)

Multiple bands corresponding to different isoforms.

• Applications: Western Blot
• Cell Tissue Type: Mouse Heart

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