TTC39A Polyclonal antibody

TTC39A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21323-1-AP

Synonyms

C1orf34, DEME 6, KIAA0452, TPR repeat protein 39A, TTC39A

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Validation Data Gallery

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  WB analysis of mouse liver using 21323-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21323-1-AP (TTC39A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21323-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21323-1-AP (TTC39A antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 21323-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21323-1-AP (TTC39A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 21323-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21323-1-AP (TTC39A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 21323-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21323-1-AP (TTC39A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 21323-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21323-1-AP (TTC39A antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 21323-1-AP

  Immunofluorescent analysis of HepG2 cells, using TTC39A antibody 21323-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HeLa using 21323-1-AP

  Immunofluorescent analysis of Hela cells, using TTC39A antibody 21323-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse liver tissue, MCF-7 cells
• Positive IHC detected in: human breast cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21323-1-AP is TTC39A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TTC39A fusion protein Ag14874
• Full Name: tetratricopeptide repeat domain 39A
• Calculated Molecular Weight: 613 aa, 70 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC004399
• Gene Symbol: TTC39A
• Gene ID (NCBI): 22996
• RRID: AB_10733645
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.