TTC30A Monoclonal antibody

TTC30A Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A11B3

Cat no : 68232-1-Ig

Synonyms

FLJ13946, FLJ77601, TPR repeat protein 30A, TTC30A

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Validation Data Gallery

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  WB analysis using 68232-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68232-1-Ig (TTC30A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68232-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68232-1-Ig (TTC30A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of hTERT-RPE1 using 68232-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using TTC30A antibody (68232-1-Ig, Clone: 1A11B3 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, green).

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Tested Applications

• Positive WB detected in: LNCaP cells, rat testis tissue, HeLa cells, HEK-293 cells, mouse testis tissue
• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68232-1-Ig targets TTC30A in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TTC30A fusion protein Ag18361
• Full Name: tetratricopeptide repeat domain 30A
• Calculated Molecular Weight: 665 aa, 76 kDa
• Observed Molecular Weight: 72-76 kDa
• GenBank Accession Number: BC042848
• Gene Symbol: TTC30A
• Gene ID (NCBI): 92104
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tetratricopeptide repeat protein 30A (TTC30A) as a component of ciliary segmentation, may be essential for cartilage differentiation and renal tubulogenesis (PMID: 34548398). It is required for polyglutamylation of axonemal tubulin and plays a role in anterograde intraflagellar transport (IFT), the process by which cilia precursors are transported from the base of the cilium to the site of their incorporation at the tip.