TRUB2 Polyclonal antibody

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• KD/KO Validated

TRUB2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 19891-1-AP

Synonyms

CLONE24922, RP11 339B21.1, TRUB2

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Validation Data Gallery

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  WB analysis using 19891-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19891-1-AP (TRUB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19891-1-AP

  WB result of TRUB2 antibody (19891-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRUB2 transfected HeLa cells.

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  IHC staining of human colon cancer using 19891-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19891-1-AP (TRUB2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 19891-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19891-1-AP (TRUB2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HT-1080 cells, HeLa cells, HepG2 cells, K-562 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

19891-1-AP targets TRUB2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRUB2 fusion protein Ag13726
• Full Name: TruB pseudouridine (psi) synthase homolog 2 (E. coli)
• Calculated Molecular Weight: 331 aa, 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC001457
• Gene Symbol: TRUB2
• Gene ID (NCBI): 26995
• RRID: AB_10640900
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	EMBO Rep; A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability.; Authors - Hana Antonicka
human	WB	Nat Cell Biol; Regulators of mitonuclear balance link mitochondrial metabolism to mtDNA expression; Authors - Nicholas J Kramer