TRPM1 Polyclonal antibody

TRPM1 Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 55111-1-AP

Synonyms

LTRPC1, Melastatin 1, MLSN, MLSN1, TRPM1

---

Validation Data Gallery

• 

  IHC staining of human lung cancer using 55111-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55111-1-AP (TRPM1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 55111-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55111-1-AP (TRPM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 55111-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 55111-1-AP (TRPM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 55111-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 55111-1-AP (TRPM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 55111-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 55111-1-AP (TRPM1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of A431 using 55111-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 55111-1-AP (TRPM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 55111-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug TRPM1 antibody (55111-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Tested Applications

• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells, A431 cells
• Positive FC (Intra) detected in: SH-SY5Y cells
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:25-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55111-1-AP targets TRPM1 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: transient receptor potential cation channel, subfamily M, member 1
• Calculated Molecular Weight: 182 kDa
• GenBank Accession Number: NM_002420
• Gene Symbol: TRPM1
• Gene ID (NCBI): 4308
• RRID: AB_2881269
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.