HTF9C Polyclonal antibody

• Featured Product
• KD/KO Validated

HTF9C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16199-1-AP

Synonyms

HTF9C, TRMT2A

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Validation Data Gallery

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  WB analysis of MCF-7 using 16199-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16199-1-AP (HTF9C antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16199-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16199-1-AP (HTF9C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16199-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16199-1-AP (HTF9C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16199-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16199-1-AP (HTF9C antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16199-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16199-1-AP (HTF9C antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16199-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16199-1-AP (HTF9C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16199-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16199-1-AP (HTF9C antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 16199-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16199-1-AP (HTF9C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 16199-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16199-1-AP (HTF9C antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HepG2 cells, mouse liver tissue
• Positive IHC detected in: human kidney tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

16199-1-AP targets HTF9C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HTF9C fusion protein Ag9112
• Full Name: TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 625 aa, 69 kDa
• Observed Molecular Weight: 42-44 kDa, 68-75 kDa
• GenBank Accession Number: BC013352
• Gene Symbol: TRMT2A
• Gene ID (NCBI): 27037
• RRID: AB_2209623
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

tRNA methyltransferase 2 homolog A (TRMT2A), also known as HTF9C is a novel cell cycle regulated protein, as associated with aggressive clinical course. HTF9C harbors a putative RNA recognition motif (RRM) in the N-terminus and the conserved uracil-C5-methyltransferase domain of the TrmA family in the C-terminus. HTF9C antibody can detect two bands about 68 kDa and 40 kDa (PMID: 20307320).

Publications

Species	Application	Title
human	WB	Nat Chem Biol; Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation.; Authors - Wei Dai ; KD Validated