TRMT12 Recombinant antibody

TRMT12 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241483H1

Cat no : 84167-3-RR

Synonyms

tRNA yW synthesizing protein 2, tRNA wybutosine-synthesizing protein 2 homolog, tRNA methyltransferase 12, TRM12, EC:2.5.1.114

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Validation Data Gallery

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  WB analysis using 84167-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84167-3-RR (TRMT12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, MCF-7 cells, U2OS cells, U-521 cells, MDA-MB-231 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84167-3-RR is TRMT12 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TRMT12 fusion protein Ag9258
• Full Name: tRNA methyltransferase 12 homolog (S. cerevisiae)
• Calculated Molecular Weight: 448 aa, 50 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC011713
• Gene Symbol: TRMT12
• Gene ID (NCBI): 55039
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.