TRIM67 Polyclonal antibody

TRIM67 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 24369-1-AP

Synonyms

TNL, TRIM67, TRIM9 like protein, tripartite motif containing 67

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Validation Data Gallery

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  WB analysis of HepG2 using 24369-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24369-1-AP (TRIM67 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 24369-1-AP is TRIM67 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM67 fusion protein Ag21632
• Full Name: tripartite motif-containing 67
• Calculated Molecular Weight: 783 aa, 84 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC157055
• Gene Symbol: TRIM67
• Gene ID (NCBI): 440730
• RRID: AB_2879510
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM67(Tripartite motif-containing protein 67) is also named as TNL and belongs to the belongs to the TRIM/RBCC family. The deduced protein contains an N-terminal RING finger domain, 2 B-boxes, a coiled-coil region, a COS box, an FN3 domain, and a B30.2 domain. TRIM67 shares 65% amino acid identity with TRIM9. This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse,pig		Int J Mol Sci; Ameliorating Effects of TRIM67 against Intestinal Inflammation and Barrier Dysfunction Induced by High Fat Diet in Obese Mice; Authors - Qihui Luo
human	IHC,IF	Ann Neurol; Detection of High-Risk Paraneoplastic Antibodies Against TRIM9 and TRIM67 proteins; Authors - Christopher M Bartley
	WB	J Agric Food Chem; Dandouchi Polypeptide Alleviates Depressive-like Behavior and Promotes Hippocampal Neurogenesis by Activating the TRIM67/NF-κB Pathway in CUMS-Induced Mice; Authors - Zhongda Wang ; KD Validated