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  • KD/KO Validated

TRIM6 Polyclonal antibody

TRIM6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11953-1-AP

Synonyms

RING finger protein 89, RNF89, TRIM6, tripartite motif containing 6



Tested Applications

Positive WB detected inHepG2 cells, COLO 320 cells, human liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11953-1-AP targets TRIM6 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM6 fusion protein Ag2556
Full Name tripartite motif-containing 6
Calculated Molecular Weight 488 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC065575
Gene Symbol TRIM6
Gene ID (NCBI) 117854
RRIDAB_874230
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TRIM6 antibody 11953-1-APDownload protocol
IHC protocol for TRIM6 antibody 11953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Oxid Med Cell Longev

TRIM6 Reduces Ferroptosis and Chemosensitivity by Targeting SLC1A5 in Lung Cancer

Authors - Ying Zhang
humanIHC

Front Oncol

Tripartite Motif Protein 6 Promotes Colorectal Cancer Cell Migration and Metastasis via SOCS2-STAT3 Signaling

Authors - Hongjian Zhao
humanWB,IHC,CoIP

J Exp Clin Cancer Res

TRIM6 promotes colorectal cancer cells proliferation and response to thiostrepton by TIS21/FoxM1.

Authors - Shuier Zheng
  • KD Validated
humanWB,IHC

J Chem Neuroanat

Silencing TRIM6 inhibits the growth and angiogenesis of gliomas by regulating VEGFA

Authors - Xin Liu
humanWB,IF,IP

J Med Virol

TRIM6 facilitates SARS-CoV-2 proliferation by catalyzing the K29-typed ubiquitination of NP to enhance the ability to bind viral genomes

Authors - Jian Zhou
  • KD Validated
human,mouseWB,IHC,CoIP

Transl Oncol

TRIM6 promotes glioma malignant progression by enhancing FOXO3A ubiquitination and degradation

Authors - Jingpeng Guo
  • KD Validated