TRIM25 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRIM25 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

25

Reviews (1)

Cat no : 12573-1-AP

Synonyms

RING-type E3 ubiquitin transferase TRIM25, RING-type E3 ubiquitin transferase, Estrogen-responsive finger protein, EFP, EC:6.3.2.n3

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Validation Data Gallery

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  WB analysis using 12573-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12573-1-AP (TRIM25 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 12573-1-AP

  IP result of anti-TRIM25 (IP:12573-1-AP, 4ug; Detection:12573-1-AP 1:600) with K-562 cells lysate 3200ug.

• 

  IHC staining of human kidney using 12573-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12573-1-AP (TRIM25 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 12573-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12573-1-AP (TRIM25 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12573-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRIM25 antibody (12573-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 12573-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRIM25 antibody (12573-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, K-562 cells, MCF-7 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 21 publications below
• IF: See 5 publications below
• IP: See 2 publications below
• CoIP: See 3 publications below

Product Information

12573-1-AP targets TRIM25 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM25 fusion protein Ag3241
• Full Name: tripartite motif-containing 25
• Calculated Molecular Weight: 630 aa, 71 kDa
• Observed Molecular Weight: 68-71 kDa
• GenBank Accession Number: BC016924
• Gene Symbol: TRIM25
• Gene ID (NCBI): 7706
• RRID: AB_2209732
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity(PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection(PMID: 17392790). TRIM25 can be detected in both the cytoplasmic and nuclear (PMID: 33966039).

Publications

Species	Application	Title
	WB	Signal Transduct Target Ther; Suppression of ITPKB degradation by Trim25 confers TMZ resistance in glioblastoma through ROS homeostasis; Authors - Yuanliang Yan
human	WB	Cell Rep; p53-responsive CMBL reprograms glucose metabolism and suppresses cancer development by destabilizing phosphofructokinase PFKP; Authors - Yingdan Huang
human	WB	Elife; Cyclophilin A-regulated ubiquitination is critical for RIG-I-mediated antiviral immune responses.; Authors - Wei Liu
human	WB	Haematologica; Hypoxia-induced long non-coding RNA DARS-AS1 regulates RBM39 stability to promote myeloma malignancy.; Authors - Jia Tong
human	WB	MBio; ISG15 Deficiency Enhances HIV-1 Infection by Accumulating Misfolded p53.; Authors - Edmund Osei Kuffour
human	WB,IF	J Virol; Robust Lys63-linked Ubiquitination of RIG-I Promotes Cytokine Eruption in Early Influenza B Virus Infection.; Authors - Jingwen Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iram (Verified Customer) (01-15-2021)

TRIM25 antibody giving a very clear and sharp expression.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC
• Cell Tissue Type: Human colon cancer cells and tissue