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  • KD/KO Validated

TRIM22 Polyclonal antibody

TRIM22 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13744-1-AP

Synonyms

GPSTAF50, RING finger protein 94, RNF94, Staf 50, STAF50, TRIM22, tripartite motif containing 22



Tested Applications

Positive IHC detected inhuman lung cancer tissue, mouse spleen tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13744-1-AP targets TRIM22 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM22 fusion protein Ag4683
Full Name tripartite motif-containing 22
Calculated Molecular Weight 498 aa, 57 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC035582
Gene Symbol TRIM22
Gene ID (NCBI) 10346
RRIDAB_2877974
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tripartite motif containing 22 (TRIM22), a member of the TRIM/RBCC family, has been reported to activate the nuclear factor-kappa B (NF-κB) pathway in unstimulated macrophage cell lines (PMID: 23818111). TRIM22 is involved in the host antiviral response, TRIM22 expression is modulated in response to several other viruses and viral antigens.TRIM22 expression is high in monocytes and early granulocytes but decreases in the lymphocyte and late granulocyte populations and is undetectable in erythroid cells (PMID: 32814880).

Protocols

Product Specific Protocols
IHC protocol for TRIM22 antibody 13744-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen.

Authors - Alice Mac Kain
humanWB,IHC

Oncotarget

TRIM22 confers poor prognosis and promotes epithelial-mesenchymal transition through regulation of AKT/GSK3β/β-catenin signaling in non-small cell lung cancer.

Authors - Li Liu
  • KD Validated
humanWB,IHC,IF

Front Cell Infect Microbiol

Constitutive TRIM22 Expression in the Respiratory Tract Confers a Pre-Existing Defence Against Influenza A Virus Infection.

Authors - Matthew Charman
  • KD Validated
humanIHC

Immunobiology

Shared biomarkers of multi-tissue origin for primary Sjogren's syndrome and their importance in immune microenvironment alterations

Authors - Tiantian Cai
humanWB

Cell Biol Int

TRIM22 knockdown suppresses chronic myeloid leukemia via inhibiting PI3K/Akt/mTOR signaling pathway.

Authors - Liyin Li
  • KD Validated
humanWB

Transl Cancer Res

FOXO3/TRIM22 axis abated the antitumor effect of gemcitabine in non-small cell lung cancer via autophagy induction.

Authors - Yang Wang
  • KD Validated