TRIM22 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRIM22 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 13744-1-AP

Synonyms

GPSTAF50, RING finger protein 94, RNF94, Staf 50, STAF50, TRIM22, tripartite motif containing 22

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Validation Data Gallery

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  IHC staining of human lung cancer using 13744-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13744-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 13744-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 13744-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13744-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13744-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human lung cancer tissue, mouse spleen tissue, mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Product Information

13744-1-AP targets TRIM22 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM22 fusion protein Ag4683
• Full Name: tripartite motif-containing 22
• Calculated Molecular Weight: 498 aa, 57 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC035582
• Gene Symbol: TRIM22
• Gene ID (NCBI): 10346
• RRID: AB_2877974
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tripartite motif containing 22 (TRIM22), a member of the TRIM/RBCC family, has been reported to activate the nuclear factor-kappa B (NF-κB) pathway in unstimulated macrophage cell lines (PMID: 23818111). TRIM22 is involved in the host antiviral response, TRIM22 expression is modulated in response to several other viruses and viral antigens.TRIM22 expression is high in monocytes and early granulocytes but decreases in the lymphocyte and late granulocyte populations and is undetectable in erythroid cells (PMID: 32814880).

Publications

Species	Application	Title
human	WB	Nat Commun; Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen.; Authors - Alice Mac Kain
human	WB,IHC	Oncotarget; TRIM22 confers poor prognosis and promotes epithelial-mesenchymal transition through regulation of AKT/GSK3β/β-catenin signaling in non-small cell lung cancer.; Authors - Li Liu ; KD Validated
human	WB,IHC,IF	Front Cell Infect Microbiol; Constitutive TRIM22 Expression in the Respiratory Tract Confers a Pre-Existing Defence Against Influenza A Virus Infection.; Authors - Matthew Charman ; KD Validated
human	IHC	Immunobiology; Shared biomarkers of multi-tissue origin for primary Sjogren's syndrome and their importance in immune microenvironment alterations; Authors - Tiantian Cai
human	WB	Cell Biol Int; TRIM22 knockdown suppresses chronic myeloid leukemia via inhibiting PI3K/Akt/mTOR signaling pathway.; Authors - Liyin Li ; KD Validated
human	WB	Transl Cancer Res; FOXO3/TRIM22 axis abated the antitumor effect of gemcitabine in non-small cell lung cancer via autophagy induction.; Authors - Yang Wang ; KD Validated