TRIM22 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRIM22 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 13744-1-AP

Synonyms

GPSTAF50, RING finger protein 94, RNF94, Staf 50, STAF50, TRIM22, tripartite motif containing 22

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Validation Data Gallery

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  IHC staining of human lung cancer using 13744-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13744-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13744-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13744-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:300-1:1200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

13744-1-AP targets TRIM22 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM22 fusion protein Ag4683
• Full Name: tripartite motif-containing 22
• Calculated molecular weight: 498 aa, 57 kDa
• Observed molecular weight: 50 kDa
• GenBank accession number: BC035582
• Gene symbol: TRIM22
• Gene ID (NCBI): 10346
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen.; Authors - Alice Mac Kain
human	WB,IHC	Oncotarget; TRIM22 confers poor prognosis and promotes epithelial-mesenchymal transition through regulation of AKT/GSK3β/β-catenin signaling in non-small cell lung cancer.; Authors - Li Liu ; KD Validated
human	WB,IHC,IF	Front Cell Infect Microbiol; Constitutive TRIM22 Expression in the Respiratory Tract Confers a Pre-Existing Defence Against Influenza A Virus Infection.; Authors - Matthew Charman ; KD Validated
human	WB	Cell Biol Int; TRIM22 knockdown suppresses chronic myeloid leukemia via inhibiting PI3K/Akt/mTOR signaling pathway.; Authors - Liyin Li ; KD Validated
human	WB	Transl Cancer Res; FOXO3/TRIM22 axis abated the antitumor effect of gemcitabine in non-small cell lung cancer via autophagy induction.; Authors - Yang Wang ; KD Validated