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  • KD/KO Validated

TRIM15 Polyclonal antibody

TRIM15 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA, IF

Conjugate

Unconjugated

Cat no : 13623-1-AP

Synonyms

RING finger protein 93, RNF93, TRIM15, tripartite motif containing 15, Zinc finger protein 178, Zinc finger protein B7, ZNF178, ZNFB7



Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13623-1-AP targets TRIM15 in WB, IHC, IP, ELISA, IF applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM15 fusion protein Ag4533
Full Name tripartite motif-containing 15
Calculated Molecular Weight 465 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC038585
Gene Symbol TRIM15
Gene ID (NCBI) 89870
RRIDAB_2303892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM15 (Tripartite motif-containing protein 15) is also named as RNF93, ZNF178, ZNFB7 and belongs to the TRIM/RBCC family. It acts as an ubiquitin-protein ligase and contributes to the endogenous restriction of retroviruses in cells.

Protocols

Product Specific Protocols
WB protocol for TRIM15 antibody 13623-1-APDownload protocol
IHC protocol for TRIM15 antibody 13623-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

TRIM15 and CYLD regulate ERK activation via lysine-63-linked polyubiquitination.

Authors - Guixin Zhu
  • KO Validated
humanWB,IP,IF

Biochim Biophys Acta Mol Basis Dis

Ubiquitin ligase TRIM15 promotes the progression of pancreatic cancer via the upregulation of the IGF2BP2-TLR4 axis

Authors - Hongkun Cai
humanIF

J Cell Sci

TRIM15 is a focal adhesion protein that regulates focal adhesion disassembly.

Authors - Pradeep D Uchil
  • KD Validated
humanIHC

iScience

Whole-genome DNA methylation and DNA methylation-based biomarkers in lung squamous cell carcinoma

Authors - Qidong Cai
humanWB

J Virol

TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity.

Authors - Uchil Pradeep D PD
  • KD Validated
humanIHC

J Cancer

Tripartite motif-containing 15 overexpression in non-small cell lung cancer is associated with poor patient prognoses.

Authors - Xiaoying Han