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TREM2 Monoclonal antibody, PBS Only

TREM2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F4H10

Cat no : 68723-1-PBS

Synonyms

TREM 2, TREM2, Trem2a, Trem2b, Trem2c

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TREM2 Monoclonal antibody (68723-1-Ig)

    WB analysis using 68723-1-Ig (same clone as 68723-1-PBS)

    Non-treated and PMA treated THP-1 cellls were subjected to SDS PAGE followed by western blot with 68723-1-Ig (TREM2 antibody) at dilution of 1:5000 incubated at room temperature for 1 hours. This data was developed using the same antibody clone with 68723-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TREM2 Monoclonal antibody (68723-1-Ig)

    IHC staining of mouse brain using 68723-1-Ig (same clone as 68723-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68723-1-Ig (TREM2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68723-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TREM2 Monoclonal antibody (68723-1-Ig)

    IHC staining of mouse brain using 68723-1-Ig (same clone as 68723-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68723-1-Ig (TREM2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68723-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68723-1-PBS targets TREM2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TREM2 fusion protein Ag6843
Full Name triggering receptor expressed on myeloid cells 2
Calculated Molecular Weight 222 aa, 25 kDa
Observed Molecular Weight26-32 kDa
GenBank Accession NumberBC032362
Gene Symbol TREM2
Gene ID (NCBI) 54209
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TREM2 (triggering receptor expressed on myeloid cells 2) is a cell surface receptor belongs to TREM family that is expressed on osteoclast, dendritic cells, macrophages, nature killers, neutrophils and microglia (PMID: 19302484). TREM2 is localized predominantly in the Golgi complex, but also shuttles to and from the cell surface in endocytic and exocytic vesicles (PMID: 16675145). TREM2 associates with DAP12 to initiate the intracellular signalling cascade via an immunoreceptor tyrosine-based activation motif (ITAM) domain and tyrosine-kinases (PMID: 23977213). TREM2 has a calculated molecular mass of ~25kDa, but apparent molecular mass of ~40kDa (PMID: 24078628). In addition, TREM2 has a soluble form termed as sTREM-2 with molecular weights ranging between about 24kDa and 40kDa (PMID: 18790823). Loss-of-function mutations in either TREM2 or DAP12 can cause Nasu-Hakola disease (PMID: 19302484).