transgelin/SM22-specific Polyclonal antibody

transgelin/SM22-specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15502-1-AP

Synonyms

SM22, SM22 alpha, SM22a, SM22alpha, Smooth muscle protein 22 alpha, TAGLN, TAGLN1, transgelin, TRANSGELIN 1

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Validation Data Gallery

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  WB analysis of mouse colon using 15502-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:2000 (under 10x lens).

• 

  IHC staining of human heart using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:2000 (under 40x lens).

• 

  IHC staining of human colon using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:2000 (under 10x lens).

• 

  IHC staining of human colon using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:2000 (under 40x lens).

• 

  IHC staining of human kidney using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:500 (under 40x lens).

• 

  IHC staining of human kidney using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human heart using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse colon tissue
• Positive IHC detected in: human heart tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:600
• Immunohistochemistry (IHC): IHC : 1:100-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

15502-1-AP targets transgelin/SM22-specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: transgelin
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: NM_003186
• Gene Symbol: transgelin/SM22
• Gene ID (NCBI): 6876
• RRID: AB_2199361
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody is specific to transgelin 1. It does not cross-react with transgelin 2 or 3.

Publications

Species	Application	Title
mouse	WB	Physiol Genomics; Inflammation and vascular smooth muscle cell dedifferentiation following carotid artery ligation; Authors - B. Paul Herring
human	WB	Front Med (Lausanne); Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer.; Authors - Fang He
human	WB	Front Genet; Bioinformatics Analysis Reveals MicroRNA-193a-3p Regulates ACTG2 to Control Phenotype Switch in Human Vascular Smooth Muscle Cells.; Authors - Weitie Wang