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TPX2 Polyclonal antibody
TPX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 11741-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, K-562 cells, HeLa cells, SMMC-7721 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human prostate cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 16 publications below |
IHC | See 4 publications below |
IF | See 5 publications below |
CoIP | See 1 publications below |
ChIP | See 1 publications below |
Product Information
11741-1-AP targets TPX2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TPX2 fusion protein Ag2334 |
Full Name | TPX2, microtubule-associated, homolog (Xenopus laevis) |
Calculated Molecular Weight | 747 aa, 86 kDa |
Observed Molecular Weight | 100 kDa |
GenBank Accession Number | BC020207 |
Gene Symbol | TPX2 |
Gene ID (NCBI) | 22974 |
RRID | AB_2208895 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).
Protocols
Product Specific Protocols | |
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WB protocol for TPX2 antibody 11741-1-AP | Download protocol |
IHC protocol for TPX2 antibody 11741-1-AP | Download protocol |
IF protocol for TPX2 antibody 11741-1-AP | Download protocol |
IP protocol for TPX2 antibody 11741-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Cell Biol WDR62 regulates spindle dynamics as an adaptor protein between TPX2/Aurora A and katanin. | ||
EMBO Rep Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis | ||
J Cell Sci Loss of protein phosphatase 6 in oocytes causes failure of meiosis II exit and impaired female fertility. | ||
J Cell Sci Importin α phosphorylation promotes TPX2 activation by GM130 to control astral microtubules and spindle orientation. | ||
Front Physiol Dysfunction of Sister Chromatids Separation Promotes Progression of Hepatocellular Carcinoma According to Analysis of Gene Expression Profiling. |