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TPX2 Polyclonal antibody
TPX2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11741-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, K-562 cells, HeLa cells, SMMC-7721 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human prostate cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells, HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 12 publications below |
IHC | See 4 publications below |
IF | See 4 publications below |
Product Information
11741-1-AP targets TPX2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TPX2 fusion protein Ag2334 |
Full Name | TPX2, microtubule-associated, homolog (Xenopus laevis) |
Calculated molecular weight | 747 aa, 86 kDa |
Observed molecular weight | 100 kDa |
GenBank accession number | BC020207 |
Gene symbol | TPX2 |
Gene ID (NCBI) | 22974 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for TPX2 antibody 11741-1-AP | Download protocol |
IHC protocol for TPX2 antibody 11741-1-AP | Download protocol |
IF protocol for TPX2 antibody 11741-1-AP | Download protocol |
IP protocol for TPX2 antibody 11741-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Cell Biol WDR62 regulates spindle dynamics as an adaptor protein between TPX2/Aurora A and katanin. | ||
EMBO Rep Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis | ||
J Cell Sci Loss of protein phosphatase 6 in oocytes causes failure of meiosis II exit and impaired female fertility. | ||
J Cell Sci Importin α phosphorylation promotes TPX2 activation by GM130 to control astral microtubules and spindle orientation. | ||
Front Physiol Dysfunction of Sister Chromatids Separation Promotes Progression of Hepatocellular Carcinoma According to Analysis of Gene Expression Profiling. |