TPM3 Polyclonal antibody

TPM3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55444-1-AP

Synonyms

Gamma tropomyosin, hscp30, hTM5, NEM1, OK/SW cl.5, TM 5, TM3, TM30, TM30nm, TPM3, TPMsk3, TRK, tropomyosin 3, Tropomyosin 5, Tropomyosin alpha 3 chain

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 55444-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55444-1-AP (TPM3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55444-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55444-1-AP (TPM3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 55444-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55444-1-AP (TPM3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 55444-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55444-1-AP (TPM3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 55444-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55444-1-AP (TPM3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 55444-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55444-1-AP (TPM3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 55444-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55444-1-AP (TPM3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 55444-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55444-1-AP (TPM3 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, HepG2 cells
• Positive IHC detected in: human colon tissue, human colon cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55444-1-AP targets TPM3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: tropomyosin 3
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 40-42 kDa
• GenBank Accession Number: NM_153649
• Gene Symbol: TPM3
• Gene ID (NCBI): 7170
• RRID: AB_2881336
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.