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  • KD/KO Validated

TOP2A-Specific Polyclonal antibody

TOP2A-Specific Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (1)





Cat no : 20233-1-AP


DNA topoisomerase 2 alpha, TOP2, TOP2A, TOP2A-Specific, TP2A

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, L02 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20233-1-AP targets TOP2A-Specific in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name topoisomerase (DNA) II alpha 170kDa
Calculated Molecular Weight 174 kDa
Observed Molecular Weight 170-177 kDa
GenBank Accession NumberNM_001067
Gene Symbol TOP2A
Gene ID (NCBI) 7153
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.


Product Specific Protocols
WB protocol for TOP2A-Specific antibody 20233-1-APDownload protocol
IHC protocol for TOP2A-Specific antibody 20233-1-APDownload protocol
IF protocol for TOP2A-Specific antibody 20233-1-APDownload protocol
IP protocol for TOP2A-Specific antibody 20233-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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