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TOP2A-Specific Polyclonal antibody
TOP2A-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 20233-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, L02 cells, A431 cells, HepG2 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human spleen tissue, mouse testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
20233-1-AP targets TOP2A-Specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||topoisomerase (DNA) II alpha 170kDa|
|Calculated molecular weight||174 kDa|
|Observed molecular weight||170-177 kDa|
|GenBank accession number||NM_001067|
|Gene ID (NCBI)||7153|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.
|Product Specific Protocols|
|WB protocol for TOP2A-Specific antibody 20233-1-AP||Download protocol|
|IHC protocol for TOP2A-Specific antibody 20233-1-AP||Download protocol|
|IF protocol for TOP2A-Specific antibody 20233-1-AP||Download protocol|
|IP protocol for TOP2A-Specific antibody 20233-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation.
Loss of phosphatase CTDNEP1 potentiates aggressive medulloblastoma by triggering MYC amplification and genomic instability
Research (Wash D C)
Magnetic Fields Affect Alcoholic Liver Disease by Liver Cell Oxidative Stress and Proliferation Regulation
J Cell Biol
LRR1-mediated replisome disassembly promotes DNA replication by recycling replisome components.
TOP2Ahigh is the phenotype of recurrence and metastasis whereas TOP2Aneg cells represent cancer stem cells in prostate cancer.
J Med Chem
Optimization of the Natural Product Calothrixin A to Discover Novel Dual Topoisomerase I and II Inhibitors with Improved Anticancer Activity.