Tom22 Polyclonal antibody

Tom22 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 11278-1-AP

Synonyms

TOMM22, MSTP065, MST065, hTom22, 1C9 2

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Validation Data Gallery

• 

  WB analysis of HeLa using 11278-1-AP

  HeLa and K-562 cells were subjected to SDS PAGE followed by western blot with 11278-1-AP (Tom22 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11278-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11278-1-AP (Tom22 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11278-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11278-1-AP (Tom22 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 11278-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11278-1-AP (Tom22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11278-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11278-1-AP (Tom22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11278-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11278-1-AP (Tom22 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa, K-562 cells, HeLa cells, mouse liver tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IF: See 3 publications below

Product Information

11278-1-AP targets Tom22 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Tom22 fusion protein Ag1797
• Full Name: translocase of outer mitochondrial membrane 22 homolog (yeast)
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 15-22 kDa
• GenBank Accession Number: BC009363
• Gene Symbol: TOMM22/Tom22
• Gene ID (NCBI): 56993
• RRID: AB_2207542
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tom22 is a central receptor component of the translocase of the outer membrane of mitochondria responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. It is showed in UniProt that phosphorylation is performed at three positions of this protein which may increase the molecular weight. Catalog#66562-1-Ig recognises  the  22 kDa  protein larger than the calculated MW which is 15 kDa. Besides, it has been reported the MV of TOMM22 is 22 kDa (PMID:19285029).

Publications

Species	Application	Title
human	WB	Cell Res; Tom20 senses iron-activated ROS signaling to promote melanoma cell pyroptosis.; Authors - Bo Zhou
human	WB,IF	Cell Discov; Dynamic O-GlcNAcylation coordinates ferritinophagy and mitophagy to activate ferroptosis.; Authors - Fan Yu
human	WB	J Genet Genomics; HPDL deficiency causes a neuromuscular disease by impairing the mitochondrial respiration.; Authors - Yu Sun
human	WB	Mol Biol Cell; Mitochondrial E3 ubiquitin ligase MARCH5 controls mitochondrial fission and cell sensitivity to stress-induced apoptosis through regulation of MiD49 protein.; Authors - Shan Xu
human	WB	J Hum Genet; Mutations in TOMM70 lead to multi-OXPHOS deficiencies and cause severe anemia, lactic acidosis, and developmental delay.; Authors - Xiujuan Wei
human	WB	EMBO Rep; UBXD8 mediates mitochondria-associated degradation to restrain apoptosis and mitophagy; Authors - Jing Zheng

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