TNPO2 Polyclonal antibody

• Featured Product
• KD/KO Validated

TNPO2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 17831-1-AP

Synonyms

TNPO 2, KPNB2B, Karyopherin beta-2b, IPO3, Importin 3

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Validation Data Gallery

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  WB analysis of fetal human brain using 17831-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17831-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17831-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17831-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 17831-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 17831-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TNPO2 antibody (17831-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

17831-1-AP targets TNPO2 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TNPO2 fusion protein Ag12229
• Full Name: transportin 2
• Calculated Molecular Weight: 887 aa, 100 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC072420
• Gene Symbol: TNPO2
• Gene ID (NCBI): 30000
• RRID: AB_10598481
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Cell Rep; Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function.; Authors - Eva-Maria Hock
human	WB,IF	Sci Rep; Transportin-2 plays a critical role in nucleocytoplasmic shuttling of oestrogen receptor-α.; Authors - Tetsuji Moriyama ; KD Validated
mouse	WB	eLife; Kap-β2/Transportin mediates β-catenin nuclear transport in Wnt signaling; Authors - Woong Y Hwang
human	WB,CoIP	Int J Biol Macromol; Membrane-bound transcription factor LRRC4 inhibits glioblastoma cell motility; Authors - Yang Liu