Cardiac Troponin T Polyclonal antibody

Cardiac Troponin T Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Conjugate

Unconjugated

Publications

61

Reviews (2)

Cat no : 15513-1-AP

Synonyms

TNNT2, cTnT, Troponin T, cardiac muscle

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Validation Data Gallery

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  WB analysis of mouse heart using 15513-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15513-1-AP (Cardiac Troponin T antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 15513-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 15513-1-AP (Cardiac Troponin T antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15513-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15513-1-AP (Cardiac Troponin T antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 15513-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15513-1-AP (TNNT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 15513-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15513-1-AP (Cardiac Troponin T antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 15513-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15513-1-AP (Cardiac Troponin T antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 15513-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15513-1-AP (Cardiac Troponin T antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 15513-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15513-1-AP (Cardiac Troponin T antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 15513-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Cardiac Troponin T antibody (15513-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 15513-1-AP

  Frozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T/cTnT (red, Cat. No 15513-1-AP), CD31/PECAM-1 (magenta), and Arl13B (green, Cat. No 66739-1-Ig) with DAPI as a counterstain for visualizing the nucleus (blue). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 555 secondary antibody. CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody. Arl13B stains primary cilia and was visualized with an Alexa Fluor 488 secondary antibody. The Image and figure legends are intellectual property of @LAF_in_the_LAB.

• 

  IF Staining of mouse heart using 15513-1-AP

  Frozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T/cTnT (magenta, Cat. No 15513-1-AP), CD31/PECAM-1 (white), and Arl13B (yellow, Cat. No 66739-1-Ig) with DAPI as a counterstain for visualizing the nucleus (cyan). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 555 secondary antibody and psedocolored to magenta.CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody and psedocolored to white. Arl13B stains primary cilia and was visualized with an Alexa Fluor 488 secondary antibody and psedocolored to yellow. The Image and figure legends are intellectual property of @LAF_in_the_LAB.

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Tested Applications

• Positive WB detected in: mouse heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue
• Positive IHC detected in: mouse heart tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue
• Positive IF-Fro detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 43 publications below
• FC: See 3 publications below

Product Information

15513-1-AP targets Cardiac Troponin T in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cardiac Troponin T fusion protein Ag7716
• Full Name: troponin T type 2 (cardiac)
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 34-40 kDa
• GenBank Accession Number: BC002653
• Gene Symbol: Cardiac Troponin T
• Gene ID (NCBI): 7139
• RRID: AB_2206563
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Nature; Brahma safeguards canalization of cardiac mesoderm differentiation.; Authors - Swetansu K Hota
pig	IF	Cell Stem Cell; Generation of a humanized mesonephros in pigs from induced pluripotent stem cells via embryo complementation; Authors - Jiaowei Wang
human	IF	Sci Transl Med; Precise genomic editing of pathogenic mutations in RBM20 rescues dilated cardiomyopathy; Authors - Takahiko Nishiyama
human	IF	ACS Nano; Intrinsic Color Sensing System Allows for Real-Time Observable Functional Changes on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.; Authors - Yiqi Gong
mouse	IF	Acta Pharm Sin B; Hippo pathway-manipulating neutrophil-mimic hybrid nanoparticles for cardiac ischemic injury via modulation of local immunity and cardiac regeneration; Authors - Qiaozi Wang
mouse	IHC	Dev Cell; A Multifunctional Protein, EWS, Is Essential for Early Brown Fat Lineage Determination.; Authors - Park Jun Hong JH

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rebecca (Verified Customer) (03-16-2022)

20ug of protein were loaded. Transference was performed at 4ºC and 90V for 2h. Antibody incubation was done ON at 4ºC.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: iPS-CM

Cardiac Troponin T Antibody Western Blot validation (1:1000 dilution) in iPS-CM (Cat no:15513-1-AP)

FH

Lianjie (Verified Customer) (07-26-2019)

This antibody works well.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Mouse embryonic heart

Cardiac Troponin T Antibody Immunohistochemistry, validation (1:50 dilution) in Mouse embryonic heart (Cat no:15513-1-AP)

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