Cardiac Troponin I Polyclonal antibody

• Featured Product
• KD/KO Validated

Cardiac Troponin I Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

35

Cat no : 21652-1-AP

Synonyms

cTnI, TNNI3

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Validation Data Gallery

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  WB analysis using 21652-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21652-1-AP (Cardiac Troponin I antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 21652-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 21652-1-AP (Cardiac Troponin I antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21652-1-AP

  WB result of Cardiac Troponin I antibody (21652-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cardiac Troponin I transfected HEK-293 cells.

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  WB analysis of mouse heart using 21652-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21652-1-AP (Cardiac Troponin I antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 21652-1-AP

  IP result of anti-Cardiac Troponin I (IP:21652-1-AP, 4ug; Detection:21652-1-AP 1:30000) with mouse heart tissue lysate 1200 ug.

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  IHC staining of mouse heart using 21652-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21652-1-AP (Cardiac Troponin I antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 21652-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21652-1-AP (Cardiac Troponin I antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 21652-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21652-1-AP (Cardiac Troponin I antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 21652-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 21652-1-AP (Cardiac Troponin I antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 21652-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Cardiac Troponin I antibody (21652-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 21652-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Cardiac Troponin I antibody (21652-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue, HEK-293 cells
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: mouse heart tissue, human heart tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 17 publications below
• IHC: See 1 publications below
• IF: See 17 publications below
• ELISA: See 1 publications below

Product Information

21652-1-AP targets Cardiac Troponin I in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cardiac Troponin I fusion protein Ag16365
• Full Name: troponin I type 3 (cardiac)
• Calculated Molecular Weight: 210 aa, 24 kDa
• Observed Molecular Weight: 24-26 kDa
• GenBank Accession Number: BC096165
• Gene Symbol: Cardiac Troponin I
• Gene ID (NCBI): 7137
• RRID: AB_2878898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Troponin I (TnI) is the inhibitory subunit of troponin; the thin filament regulatory complex that confers calcium sensitivity to striated muscle actomyosin ATPase activity. Cardiac Troponin I (cTnI) is encoded by the TNNI3 gene located on chromosome 19 in humans.

What is the molecular weight of cTnI?

The molecular weight of cTnI is 24 kDa.

What is the tissue specificity of cTnI?

cTnI is produced exclusively by cardiac muscle.

What are the post-translational modifications of cTnI?

cTnI at Ser-23 and Ser-24 under adrenergic stimulation enhances the relaxation of cardiac muscle.

What is the role of cTnI in disease?

cTnI is considered to be one of the most specific and sensitive markers of myocardial damage such as acute myocardial infarction (AMI), as detection of cTnI in serum is widely accepted as a diagnostic tool (PMID: 15452154). Defects in TNNI3 lead to cardiomyopathy familial hypertrophic type 7 (CMH7); an inherited heart disorder characterized by ventricular hypertrophy (PMID: 8205619). Defects in TNNI3 can also cause cardiomyopathy familial restrictive type I (RCM1) (PMID: 20617149) and cardiomyopathy dilated type 2A (CMD2A) (PMID: 12531876). These disorders are characterized by the impaired filling of ventricles or ventricular dilation respectively.

Publications

Species	Application	Title
human	IF	EBioMedicine; Elevated myocardial SORBS2 and the underlying implications in left ventricular noncompaction cardiomyopathy.; Authors - Chunyan Li
mouse	IF	Transl Res; Epididymal white adipose tissue promotes angiotensin II-induced cardiac fibrosis in an exosome-dependent manner.; Authors - Mengqi Su
		Biosens Bioelectron; Improving the reproducibility, accuracy, and stability of an electrochemical biosensor platform for point-of-care use.; Authors - Lung-Chieh Chen
mouse	WB	Theranostics; miR-17-3p Contributes to Exercise-Induced Cardiac Growth and Protects against Myocardial Ischemia-Reperfusion Injury.; Authors - Jing Shi
mouse	IF	Arterioscler Thromb Vasc Biol; Platelet Internalization Mediates Ferroptosis in Myocardial Infarction; Authors - Shuo Miao
mouse	IF	EBioMedicine; Microtubule associated protein 4 phosphorylation leads to pathological cardiac remodeling in mice.; Authors - Lingfei Li

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