TNNI2 Polyclonal antibody

TNNI2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11634-1-AP

Synonyms

AMCD2B, DA2B, FSSV, fsTnI, TNNI2, Troponin I fast skeletal muscle

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Validation Data Gallery

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  WB analysis using 11634-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11634-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 11634-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 11634-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 11634-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11634-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 11634-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 11634-1-AP (TNNI2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 11634-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 11634-1-AP (TNNI2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse skeletal muscle using 11634-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11634-1-AP (TNNI2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 11634-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11634-1-AP (TNNI2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, human kidney tissue, human spleen tissue, human skeletal muscle tissue, rat skeletal muscle tissue
• Positive IHC detected in: mouse skeletal muscle tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11634-1-AP targets TNNI2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TNNI2 fusion protein Ag2208
• Full Name: troponin I type 2 (skeletal, fast)
• Calculated Molecular Weight: 182 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC032148
• Gene Symbol: TNNI2
• Gene ID (NCBI): 7136
• RRID: AB_2206109
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.